GC-MS and HPLC analyses of phytochemical compounds from withania somnifera L. leaves extract

Abstract EN

In the present work, the shrub W.

somnifera was used as a source for the extraction of bioactive molecules.

leaves were extracted by ethanol and analyzed by Gas chromatography-mass spectrometry (GC-MS) and high-performance liquid chromatography (HPLC).

by GC-MS analysis, the highest identified compounds were 2,6-dimethyl-N-(2-methyl-α-phenyl benzyl) aniline, 4-amino heptane, n-undecanophenone, 2-cyclohexyl-4-hydroxymethyl-octahydrobenzo [E] [1,2] oxazine-3-carbonitrile, and octadecanoic acid 3-octadecyloxypropyl ester, with percentages of 19.43%, 7.51%, 4.47%, 4.34%, and 3.89%, respectively.

HPLC analysis identified quercetin and 7-hydroxyflavone with concentrations of 12.36 and 15.14 MG / ML, respectively, as two flavonoid compounds.

this work showed that W.

somnifera leaf extract owns several bioactive compounds that could be useful and have different applications, such as antimicrobial activities.