Evaluation of rose bengal test, gel immunodiffusion with native 132 hapten, and indirect elisa in diagnosis of brucellosis in infected and vaccinated sheep

Abstract EN

^ serological assays were evaluated for the diagnosis of brucellosis the Rose Bengal plate test as a screening test (RBPT), agar gel immunodiffusion with Native Hapten test (AGID-NH) and an i * enzyme-linked immunosorbent assay (iELISA).

For optimal sensi/^1 RBPT had to be used with sera: antigen at a 3:1 dilution.

With the^1 from B.

melitensis culture-positive sheep, the sensitivity was lOOo/^ RBPT, and iELISA; while, it was 93.8% for AGID-NH test.

On other hand specificity was 100% when testing the sera from brucella-free sheep.

Larger discrepancies among the results serological tests were obtained with sera from sheep of areas wher brucellosis is endemic.

Sheep vaccinated with B.

melitensis Re 1 subcutaneously and conjunctively showed negative result in all tests after twelve and six months respectively.

The proportion of sheep giv; a positive reaction after vaccination decreased faster in AGID-NH test than in other tests.

The using of RBPT or ELISA with combination with AGID-NH is the best choice for diagnosis of infected and vaccinated sheep