Diagnosis of brucellosis in lactating ewes using milk-elisa

Abstract EN

M'lk and blood samples collected from 731 lactating ewes were xamined for brucellosis using milk-ELISA, serum-ELISA, RBPT, SAT d MRT.

Of these ewes, 150 were from brucellosis free sheep flock, /hich were found negative in all tests used.

While, of the remaining 581 ejves, 111 were positive in serum-ELISA, 107 in milk-ELISA, 96 in RBPT, 85 in SAT, 48 in MRT and B.melitensis biovar 3 was isolated from the milk of 39 infected ewes.

Of the 111 serum-ELISA positive ewes, 101 were positive and 10 were negative in milk-ELISA, whereas of the 107 milk-ELISA positive ewes, 6 ewes were negative in serum- ELISA.

All together, 101 ewes were positive and 464 were negative in both the assays, while 16 ewes yield variable results.

The se • 1HF positive predictive value in the infected flocks were 94.390/ Sltlv'ty^B| respectively.

The milk-ELISA for diagnosing brucellosis see^s attractive alternative of serum-ELISA particularly in the lactat;S to ^ an U