Serological investigations for detection of chlamydia infection in aborted cattle in Assiut Govemorate

Abstract EN

Serological study was carried out on the chlamydial related infect' cattle at Assiut Governorate.

Eighty five cows in different localif ^ 'n examined (sixty cows with reproductive disorders in four farms as$ ^ group and twenty five clinically healthy cows in three different fa^^- a control group).

The diseased tested cows were divided into 11!35 subgroups; the first tested subgroup (20 cows) with age 3-5 years- second tested subgroup (25 cows) with age 5-8 years and the third tp ^ subgroup (15 cows) with age more than eight years.

Blood samples w taken from each animal with different ages (three years to more th ^ eight years).

Samples were examined for both IgG and IgM Chlamydia antibodies with an indirect ELISA.

Twenty-one cows of the total examined cows (85) were serologically positive for both IgG and Ig^ Chlamydia antibodies with a percentage 24.7% (33.3% of the diseased tested cows and 4% of the clinically healthy cows).

The serological investigation referred that the infection was higher in diseased tested cows at age 3-5 years; where 35% of these tested cows were serologically positive for both IgG and IgM Chlamydia antibodies.

Also seropositivity was 32% and 33.3% of the diseased tested cows in the second (5-8 years) and the third (>8 years) tested subgroups, respectively.

It can be concluded that the enzyme immunoassay (ELISA) was easily to perform and can used as rapid screening test for detection of recent, latent chlamydial infections and apparently healthy cows