تحريض المقاومة الجهازية في نباتات البندورة-الطماطم ضد فيروس تجعد و اصفرار أوراق البندورة-الطماطم في ظروف الزراعة المحمية باستخدام عزلة بكتيرية محلية التابعة للنوع Bacillus subtilis

Abstract EN

This study was conducted at the Agricultural Scientific Research Center in Lattakia Governorate in a plastic house during the 2020/2021 growing season to evaluate the efficiency of the native bacterial isolate B.Ra.217 of Bacillus subtilis, in reducing infection of tomato plants with Tomato yellow leaf curl virus (TYLCV), by treating tomato seeds and later watering the seedlings with a suspension of the tested bacteria at a concentration of 1x109/ml, and then measuring incidence (%) and severity of virus infection, and estimating peroxidase enzyme activity.

The results showed that 30 days after inoculation with tomato leaf curl virus, a decrease in virus infection rate and severity in virus-infected and bacteria-treated plants compared with virus-infected and untreated control plants was observed.

The reduction in disease incidence and severity of infection reached 26.67% and 34.28%, respectively, with significant differences between the treatments.

In addition, the activity of peroxidase enzyme 7 days after infection with the virus showed an increase in plants treated with bacteria only (0.1342 ^mol/mg) and those treated with bacteria and virus-infected (0.0913 |imol/mg), compared with the healthy control plants (0.0958 |imol/mg), and virus-infected and untreated with bacteria (0.0570 ^mol/mg).

The results also showed 15 days after infection that the enzyme activity was higher in plants treated with bacteria only (0.1592 ^mol/mg) compared with the healthy control (0.1415 ^mol/mg) with significant differences, and also the differences were significant with the untreated infected plants (0.1002 ^mol/mg), and with inoculated and treated plants (0.1372 ^mol/mg).

Thus, this bacterial isolate may have an important applied role in enhancing tomato plant resistance to the virus and consequently reducing its damage.