تقويم نشاط إنزيم التاناز المنتج بفعل الفطرين Trichoderma citrinoviride و Trichoderma brevicompactu المعزولين من ترب حراجية سورية على تفكيك التانينات

Abstract EN

This study was conducted during the period 2019–2020 to identify Trichoderma spp.

isolated from woody soils and assessing their efficacy for biodegradation of tannins through tannase enzyme activity produced.

Results obtained confirmed the presence of two species; Trichoderma citrinoviride, which was isolated from Maysalon area near Damascus, and characterized by yellowish green colony with dense growth of spores at the center of the colony, and Trichoderma brevicompactum isolated from the Balluran area near Lattakia characterized by yellow colonies with concentric rings.

Trichoderma citrinoviride had higher biodegradation activity, measured by degrading different tannins concentrations (2, 4, 6%) collected from Queircus coccifera from Bmelka area in Tartous in liquid medium and led to 85, 87 and 90% degradation, for the three concentrations, respectively, following 12 days incubation.

The activity of the produced tannase was measured to be 37.9 units/mg.

Whereas, the decomposition rate of the three tannin concentrations by Trichoderma brevicompactum reached 67, 80 , 89%, respectively, again after 12 days of incubation, with enzyme activity measured to be 35.2 units/mg.