Designing a highly immunogenic multi epitope based subunit vaccine against bacillus cereus

Abstract EN

Objective: Serious non-gastrointestinal-tract infections and food poisoning are caused by Bacillus cereus.

Vaccination against B.

cereus is very important.

The aim of this study was to identify and analyze B and T cell epitopes for chromate transporter protein of the bacteria.

Methods: Multiple sequence alignment with the Clustal Omega method was used to identify conserved regions and Geneious Prime was used to produce a consensus sequence.

T and B cell epitopes were predicted by various computational tools from the NetCTL and Immune Epitope Database (IEDB), respectively.

Results: Altogether, 6 HTL cells and 11 CTL epitopes were predicted.

This vaccine’s molecular docking is done with Patch Dock and LigPlot to verify interactions.

The immune server (C-IMMSIM) was used to develop In silico immune response in order to assess the multi-epitope vaccine’s immunogenic profile.

Conclusion: We designed universal vaccine against B.

cereus responsible for food poisoning.

The disease may be avoided with the aid of the proposed epitope-based vaccine.