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A unique allele variant at STR locus D2S1338 in a paternity testing case
Other Title(s)
متغير أليلي فريد في الموقع الوراثي D2S1338 في قضية اختبار أبوة
Joint Authors
Amjad, Muhammad
Tahir, Muhammad Ashraf
Ali, Sadaqat
Siddique, Nasir
Ahmad, Qadi Laeeque
Ahmad, Sajjad
Source
Arab Journal of Forensic Sciences and Forensic Medicine
Issue
Vol. 3, Issue 1 (30 Jun. 2021), pp.109-117, 9 p.
Publisher
Naif Arab University for Security Sciences Arab Society for Forensic Sciences and Forensic Medicine
Publication Date
2021-06-30
Country of Publication
Saudi Arabia
No. of Pages
9
Main Subjects
Abstract EN
Relationship testing through DNA profiling may undesirably be affected by the rare allele variants, tri-allelic patterns and null alleles.
Therefore, it is vital to report such anomalies.
We report a paternity testing instance in a sexual assault case studied at Punjab Forensic Science Agency (PFSA), Lahore, Pakistan showing a unique allele variant in mother and child.
DNA was extracted from the buccal swabs of reference samples using organic extraction method.
DNA profiling was done for 15 autosomal STRs and amelogenin using Identifiler Plus kit.
A novel out of marker range (OMR) allele variant between STR Loci D16S539 and D2S1338 was observed in the DNA profiles of victim and her child.
At STR locus D2S1338, twenty-one different allele variants are listed at STRBase ranging from 11 to 28.
The allele variant observed in this case study appeared at less than the marker range (< D2S1338) with a size of 297.50 bp.
The novel variant OMR allele at D2S1338 was calculated as allele 13 with the help of allelic ladder.
Moreover, this allele variant (allele 13 at locus D2S1338) was present in only two other distinct DNA profiles in PFSA DNA database, out of a total 10,125 profiles.
The overall frequency of this unique allele variant was 3 in 10,125 unrelated individuals with occurrence frequency of 0.000296.
According to our limited knowledge, it is the first report of a novel OMR allele variant at D2S1338 within the Pakistani population.
American Psychological Association (APA)
Ahmad, Sajjad& Ali, Sadaqat& Siddique, Nasir& Ahmad, Qadi Laeeque& Amjad, Muhammad& Tahir, Muhammad Ashraf. 2021. A unique allele variant at STR locus D2S1338 in a paternity testing case. Arab Journal of Forensic Sciences and Forensic Medicine،Vol. 3, no. 1, pp.109-117.
https://search.emarefa.net/detail/BIM-1414307
Modern Language Association (MLA)
Ahmad, Sajjad…[et al.]. A unique allele variant at STR locus D2S1338 in a paternity testing case. Arab Journal of Forensic Sciences and Forensic Medicine Vol. 3, no. 1 (2021), pp.109-117.
https://search.emarefa.net/detail/BIM-1414307
American Medical Association (AMA)
Ahmad, Sajjad& Ali, Sadaqat& Siddique, Nasir& Ahmad, Qadi Laeeque& Amjad, Muhammad& Tahir, Muhammad Ashraf. A unique allele variant at STR locus D2S1338 in a paternity testing case. Arab Journal of Forensic Sciences and Forensic Medicine. 2021. Vol. 3, no. 1, pp.109-117.
https://search.emarefa.net/detail/BIM-1414307
Data Type
Journal Articles
Language
English
Notes
Record ID
BIM-1414307