Potential cytotoxicity of silver nanoparticles : Stimulation of autophagy and mitochondrial dysfunction in cardiac cells
Joint Authors
Khan, Azmat Ali
al-Anzi, Amir Mayuf
al-Sayf, Nawwaf Abd al-Aziz
Sayyid, Ahmad Y. A.
Bhat, Mashuq Ahmad
Source
Saudi Journal of Biological Sciences
Issue
Vol. 28, Issue 5 (31 May. 2021), pp.2762-2771, 10 p.
Publisher
Publication Date
2021-05-31
Country of Publication
Saudi Arabia
No. of Pages
10
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
In the present study, we elucidated the potential cytotoxicity of AgNPs in H9c2 rat cardiomyoblasts and assessed the underlying toxicological manifestations responsible for their toxicity thereof.
The results indicated that the exposure of AgNPs to H9c2 cardiac cells decreased cell viability in a dose-dependent manner and caused cell cycle arrest followed by induction of apoptosis.
The AgNPs treated cardiac cells showed a generation of reactive oxygen species (ROS) and mitochondrial dysfunction where mitochon- drial ATP was reduced and the expression of AMPK1a increased.
AgNPs also induced ROS-mediated autophagy in H9c2 cells.
There was a significant time-dependent increase in intracellular levels of Atg5, Beclin1, and LC3BII after exposure to AgNPs, signifying the autophagic response in H9c2 cells.
More importantly, the addition of N-acetyl-L-cysteine (NAC) inhibited autophagy and significantly reduced the cytotoxicity of AgNPs in H9c2 cells.
The study highlights the prospective toxicity of AgNPs on cardiac cells, collectively signifying a potential health risk.
American Psychological Association (APA)
Khan, Azmat Ali& al-Anzi, Amir Mayuf& al-Sayf, Nawwaf Abd al-Aziz& al-Anzi, Muhammad Matir& Sayyid, Ahmad Y. A.& Bhat, Mashuq Ahmad. 2021. Potential cytotoxicity of silver nanoparticles : Stimulation of autophagy and mitochondrial dysfunction in cardiac cells. Saudi Journal of Biological Sciences،Vol. 28, no. 5, pp.2762-2771.
https://search.emarefa.net/detail/BIM-1414618
Modern Language Association (MLA)
al-Anzi, Muhammad Matir…[et al.]. Potential cytotoxicity of silver nanoparticles : Stimulation of autophagy and mitochondrial dysfunction in cardiac cells. Saudi Journal of Biological Sciences Vol. 28, no. 5 (2021), pp.2762-2771.
https://search.emarefa.net/detail/BIM-1414618
American Medical Association (AMA)
Khan, Azmat Ali& al-Anzi, Amir Mayuf& al-Sayf, Nawwaf Abd al-Aziz& al-Anzi, Muhammad Matir& Sayyid, Ahmad Y. A.& Bhat, Mashuq Ahmad. Potential cytotoxicity of silver nanoparticles : Stimulation of autophagy and mitochondrial dysfunction in cardiac cells. Saudi Journal of Biological Sciences. 2021. Vol. 28, no. 5, pp.2762-2771.
https://search.emarefa.net/detail/BIM-1414618
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 2770-2771
Record ID
BIM-1414618