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Synthesis and cytotoxic activity of organotin(IV) diallyldithiocarbamate compounds as anticancer agent towards colon adenocarcinoma cells (HT-29)
Joint Authors
Awang, Normah
Haezam, Farah Natasha
Kamal al-Din, Nur Farhanah
Muhammad, Rapidah
Source
Saudi Journal of Biological Sciences
Issue
Vol. 28, Issue 5 (31 May. 2021), pp.3160-3168, 9 p.
Publisher
Publication Date
2021-05-31
Country of Publication
Saudi Arabia
No. of Pages
9
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
Context: Diphenyltin(IV) diallyldithiocarbamate compound (Compound 1) and triphenyltin(IV) dial- lyldithiocarbamate compound (Compound 2) are two newly synthesised compounds of organotin(IV) with diallyldithiocarbamate ligands.
Objective: To assess the cytotoxic effects of two synthesised compounds against HT-29 human colon ade- nocarcinoma cells and human CCD-18Co normal colon cells.
Materials and methods: Two successfully synthesised compounds were characterised using elemental (carbon, hydrogen, nitrogen, and sulphur) analysis, Fourier-Transform Infrared (FTIR), and 1 H, 13C 119Sn Nucleus Magnetic Resonance (NMR) spectroscopies.
The single-crystal structure of both compounds was determined by X-ray single-crystal analysis.
The cytotoxicity of the compounds was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazholium bromide (MTT) assay upon 24 h of treatment.
While the mode of cell death was determined based on the externalisation of phosphatidylserine using a flow cytometer.
Results: The elemental analysis data of the two compounds showed an agreement with the suggested for- mula of (C6H5)2Sn[S2CN(C3H5)2]2 for Compound 1 and (C6H5)3Sn[S2CN(C3H5)2] for Compound 2.
The two major peaks of infrared absorbance, i.e., m(C = N) and m(C = S) were detected at the range of 1475– 1479 cm1 and 972–977 cm1 , respectively.
The chemical shift of carbon in NCS2 group for Compound 1 and 2 were found at 200.82 and 197.79 ppm.
The crystal structure of Compound 1 showed that it is six coordinated and crystallised in monoclinic, P21/c space group.
While the crystal structure of Compound 2 is five coordinated and crystallised in monoclinic, P21/c space group.
The cytotoxicity (IC50) of the two compounds against HT-29 cell were 2.36 lM and 0.39 lM.
Meanwhile, the percentage of cell death modes between 60% and 75% for compound 1 and compound 2 were mainly due to apop- tosis, suggesting that both compounds induced growth arrest.
American Psychological Association (APA)
Haezam, Farah Natasha& Awang, Normah& Kamal al-Din, Nur Farhanah& Muhammad, Rapidah. 2021. Synthesis and cytotoxic activity of organotin(IV) diallyldithiocarbamate compounds as anticancer agent towards colon adenocarcinoma cells (HT-29). Saudi Journal of Biological Sciences،Vol. 28, no. 5, pp.3160-3168.
https://search.emarefa.net/detail/BIM-1415269
Modern Language Association (MLA)
Haezam, Farah Natasha…[et al.]. Synthesis and cytotoxic activity of organotin(IV) diallyldithiocarbamate compounds as anticancer agent towards colon adenocarcinoma cells (HT-29). Saudi Journal of Biological Sciences Vol. 28, no. 5 (2021), pp.3160-3168.
https://search.emarefa.net/detail/BIM-1415269
American Medical Association (AMA)
Haezam, Farah Natasha& Awang, Normah& Kamal al-Din, Nur Farhanah& Muhammad, Rapidah. Synthesis and cytotoxic activity of organotin(IV) diallyldithiocarbamate compounds as anticancer agent towards colon adenocarcinoma cells (HT-29). Saudi Journal of Biological Sciences. 2021. Vol. 28, no. 5, pp.3160-3168.
https://search.emarefa.net/detail/BIM-1415269
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 3168
Record ID
BIM-1415269