Production of enriched in B vitamins biomass of Yarrowia lipolytica grown in biofuel waste

Abstract EN

Yarrowia lipolytica as an oleaginous yeast is capable of growing in various non-conventional hydrophobic substrate types, especially industrial wastes.

In this study, the content of thiamine (vitamin B1), ribofla- vin (vitamin B2), pyridoxine (vitamin B6), biotin (vitamin B7) and folic acid (vitamin B9) in the wet bio- mass of Y.

lipolytica strains cultivated in biofuel waste (SK medium), compared to the standard laboratory YPD medium, was assessed.

Additionally, the biomass of Y.

lipolytica A-101 grown in biofuel waste (SK medium) was dried and examined for B vitamins concentration according to the recommended microbial methods by AOAC Official Methods.

The mean values of these vitamins per 100 g of dry weight of Y.

lipoly- tica grown in biofuel waste (SK medium) were as follows: thiamine 1.3 mg/100 g, riboflavin 5.3 mg/100 g, pyridoxine 4.9 mg/100 g, biotin 20.0 mg/100 g, and folic acid 249 mg/100 g.

We have demonstrated that the dried biomass is a good source of B vitamins which can be used as nutraceuticals to supplement human diet, especially for people at risk of B vitamin deficiencies in developed countries.

Moreover, the biodegradation of biofuel waste by Y.

lipolytica is desired for environmental protection.