The stingless bee honey protects against hydrogen peroxide-induced oxidative damage and lipopolysaccharide-induced inflammation in vitro
Joint Authors
Shahar, Suzana
Ooi, Theng Choon
Sharif, Razinah
Yacub, Malisanurhidayu
Rajab, Nur Fadilah
Source
Saudi Journal of Biological Sciences
Issue
Vol. 28, Issue 5 (31 May. 2021), pp.2987-2994, 8 p.
Publisher
Publication Date
2021-05-31
Country of Publication
Saudi Arabia
No. of Pages
8
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
Oxidative stress, DNA damage, and unresolved inflammation are the predisposing factors of many chronic and degenerative diseases, including cancer.
Stingless bee honey (SBH) is recognized to have high medic- inal value by traditional medicine practitioners and has been used to treat various illnesses traditionally.
This study aimed to determine the antioxidant, anti-inflammatory, and genoprotective effects of SBH byusing in vitro cell culture models.
The sugar content, total phenolic content, radical scavenging activity, and ferric reducing antioxidant power (FRAP) of SBH were determined in this study.
Then, the protective effect of SBH against hydrogen peroxide (H2O2)-induced cell death and DNA damage was studied by using WIL2-NS human lymphoblastoid cell line, while the lipopolysaccharide (LPS)-induced RAW 264.7 murine macrophages cell line was used to study the anti-inflammatory effects of SBH.
Results from this present study showed that the major sugar contents of SBH were fructose (19.39 + 0.01%) and glucose (14.03 ± 0.
03%).
Besides, the total phenolic content, the radical scavenging activity, and the FRAP value of SBH were 15.38 ± 0.02 mg GAE/100 g of honey, 34.04 ± 0.21%, and 206.77 + 1.76 lM AAE/100 g honey respectively.
Pretreatment with SBH protected WIL2-NS cells from H2O2-induced cell death and DNA damage (p < 0.001).
Moreover, SBH was also able to attenuate the production of nitric oxide by inhibiting the expression of inducible nitric oxide synthase in LPS-induced RAW 264.7 cells (p < 0.001).
In conclusion, SBH is rich in total phenolic content and possesses strong antioxidant, anti-inflammatory, and genopro- tective properties.
Our current findings suggest that SBH might be useful in the prevention and treatment of many diseases caused by oxidative stress and inflammation assuming the observed effects are also achievable in vivo.
American Psychological Association (APA)
Ooi, Theng Choon& Yacub, Malisanurhidayu& Rajab, Nur Fadilah& Shahar, Suzana& Sharif, Razinah. 2021. The stingless bee honey protects against hydrogen peroxide-induced oxidative damage and lipopolysaccharide-induced inflammation in vitro. Saudi Journal of Biological Sciences،Vol. 28, no. 5, pp.2987-2994.
https://search.emarefa.net/detail/BIM-1415284
Modern Language Association (MLA)
Ooi, Theng Choon…[et al.]. The stingless bee honey protects against hydrogen peroxide-induced oxidative damage and lipopolysaccharide-induced inflammation in vitro. Saudi Journal of Biological Sciences Vol. 28, no. 5 (2021), pp.2987-2994.
https://search.emarefa.net/detail/BIM-1415284
American Medical Association (AMA)
Ooi, Theng Choon& Yacub, Malisanurhidayu& Rajab, Nur Fadilah& Shahar, Suzana& Sharif, Razinah. The stingless bee honey protects against hydrogen peroxide-induced oxidative damage and lipopolysaccharide-induced inflammation in vitro. Saudi Journal of Biological Sciences. 2021. Vol. 28, no. 5, pp.2987-2994.
https://search.emarefa.net/detail/BIM-1415284
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 2993-2994
Record ID
BIM-1415284