![](/images/graphics-bg.png)
Molecular determination of van genes among clinical isolates of enterococci at a hospital setting
Joint Authors
George, Siju Kankalil
Suseela, M. R.
al-Safi, Salih
al-Naji, al-Muizz Ali
al-Naam, Yasir Abd al-Aziz
Adam, Ahmad Adlan Muhammad
Yaqub, Ashli Mari
al-Maqati, Thikra
Ks, Harish Kumar
Source
Saudi Journal of Biological Sciences
Issue
Vol. 28, Issue 5 (31 May. 2021), pp.2895-2899, 5 p.
Publisher
Publication Date
2021-05-31
Country of Publication
Saudi Arabia
No. of Pages
5
Main Subjects
Sociology and Anthropology and Social Work
Abstract EN
Vancomycin-resistant enterococci (VRE) poses a formidable challenge to public health due to its inherent resistance to multiple antibiotics coupled with the ability to transfer genetic determinants to dangerous pathogens like Methicillin-resistant Staphylococcus aureus (MRSA).
The purpose of this study was to investigate the incidence of vancomycin resistance in enterococci among clinical isolates at a tertiary care military hospital in the eastern region of Saudi Arabia and to detect van genes using multiplex-PCR.
Overall, 246 isolates of enterococci were collected from various clinical specimens.
The isolates were identified, and antimicrobial susceptibility testing was done using the Vitek 2 system.
Multiplex PCR was performed on the VRE isolates, thus identified to determine the van genes harbored.
A total of 15 VRE were identified, of which 14 (93.3%) were Enterococcus faecium, and 1(6.7%) was Enterococcus cas- seliflavus with intrinsic vanC resistance.
Of the 14 vancomycin-resistant Enterococcus faecium, 8 (57.1%) harbored vanB genes, while 6 (42.8%) harbored vanA genes.
All the VRE were susceptible to linezolid and tigecycline.
Our study detected a low prevalence (6.1%) of VRE among clinical isolates of enterococci and that the vanB gene predominates in such strains.
Susceptibility profiles indicated that linezolid and tigecycline are still effective against these multidrug-resistant pathogens.
Pus specimens yielded the highest percentage (53.3%) of isolates from which VRE was obtained, and this finding is novel among studies done in Saudi Arabia.
American Psychological Association (APA)
George, Siju Kankalil& Suseela, M. R.& al-Safi, Salih& al-Naji, al-Muizz Ali& al-Naam, Yasir Abd al-Aziz& Adam, Ahmad Adlan Muhammad…[et al.]. 2021. Molecular determination of van genes among clinical isolates of enterococci at a hospital setting. Saudi Journal of Biological Sciences،Vol. 28, no. 5, pp.2895-2899.
https://search.emarefa.net/detail/BIM-1419134
Modern Language Association (MLA)
George, Siju Kankalil…[et al.]. Molecular determination of van genes among clinical isolates of enterococci at a hospital setting. Saudi Journal of Biological Sciences Vol. 28, no. 5 (2021), pp.2895-2899.
https://search.emarefa.net/detail/BIM-1419134
American Medical Association (AMA)
George, Siju Kankalil& Suseela, M. R.& al-Safi, Salih& al-Naji, al-Muizz Ali& al-Naam, Yasir Abd al-Aziz& Adam, Ahmad Adlan Muhammad…[et al.]. Molecular determination of van genes among clinical isolates of enterococci at a hospital setting. Saudi Journal of Biological Sciences. 2021. Vol. 28, no. 5, pp.2895-2899.
https://search.emarefa.net/detail/BIM-1419134
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 2899
Record ID
BIM-1419134