أمثلة ظروف استخلاص المركبات الفينولية من قشور البرتقال و دراسة خصائصها المضادة للأكسدة

Abstract EN

The conditions for extraction phenolic compounds from dried sweet orange peel (Citrus sinensis) at 40°C for 48 hour were optimized using three solvents (water, absolute ethanol and mixture of water ethanol at the ratio of (50:50) at three different temperature (30, 50, 70°C) for 2, 3, 4 h by applying the statistical design Response Surface Methodology (RSM).The statistical analysis showed that the highest concentration of phenolic can be extracted from orange peel (1157.52 mg / 100 g) using the mixture of ethanol :water at 36°C for 2h,while the highest concentration of phenolic compounds can be obtained using ethanol (1152.76 mg/100 g) at 70°C for 2 hours, whereas distilled water gave the highest concentration of phenols (750.31 mg/100 g) at 30°C for 4 hours.

The concentration of flavonoids in the previous three extracts was 519.6, 279.83 and 35.58 mg / 100 g, respectively.

The inhibition percentage of DPPH was 63.58% in the ethanol: water extract followed by the ethanol extract (35.69%) and water extract (21.05%).

The values of antioxidant activity by FRAP method were of 5.38, 4.55 and 1.47 meq ascorbic acid /100g for three extracts respectively.