Investigating the effects of theophylline on motility, hyperactivity, and acrosome reaction of spermatozoa in rams

Joint Authors

Zaki, K.
Atiyyah, Khalid Hafiz al-Shahhat Muhammad
Taysir, M. I.
Badr, M. R.

Source

Veterinary Medicine and Public Health Journal

Issue

Vol. 1, Issue 3 (30 Sep. 2020), pp.81-84, 4 p.

Publisher

Refaad Center for Studies and Research

Publication Date

2020-09-30

Country of Publication

Jordan

No. of Pages

4

Main Subjects

Veterinary Medicine

Abstract EN

The present study was conducted to evaluate the effect of in vitro treatment of ejaculated ram spermatozoa with different levels of theophylline at various incubation times on motility, hyperactivity (HA), and acrosome reaction (AR).

Semen was collected from Barki rams (n=40 ejaculates) using an artificial vagina twice a week.

Collected semen were pooled and subjected to swim up technique in modified sperm Tyrode's albumin lactate pyruvate (S-TALP) medium supplemented with different levels of theophylline (0.01, 0.02, 0.03, 0.04, and 0.05 mg/ml) at different incubation times (0, 1,2,3 and 4 hours).

The following parameters were examined: motility, hyperactivity (HA), and acrosome reaction (AR %).

The results revealed that the highest motility was noticed in spermatozoa treated with 0.05 mg/ml theophylline (82.53±1.34%) immediately after dilution.

While the addition of 0.01 mg/ml theophylline resulted in a significant (P<0.05) increase in HA% than those obtained in control (untreated) after 3 hours of incubation (5.04±0.26 vs.0.8±0.2).

On other hand, a significant (P<0.05) increase in total AR% was concomitant to the increase in the concentration of theophylline with a maximum achieved at 0.05 mg/ml at 3- and 4-hours post-incubation (49.2±1.8; 51.2±2.5, respectively).

In conclusion, under our experimental conditions, treatment of ram spermatozoa with 0.05 mg/ml of theophylline for 3-4 hours was considered the best concentration of theophylline to be used for in vitro induction of acrosome reaction.

American Psychological Association (APA)

Atiyyah, Khalid Hafiz al-Shahhat Muhammad& Taysir, M. I.& Badr, M. R.& Zaki, K.. 2020. Investigating the effects of theophylline on motility, hyperactivity, and acrosome reaction of spermatozoa in rams. Veterinary Medicine and Public Health Journal،Vol. 1, no. 3, pp.81-84.
https://search.emarefa.net/detail/BIM-1426843

Modern Language Association (MLA)

Atiyyah, Khalid Hafiz al-Shahhat Muhammad…[et al.]. Investigating the effects of theophylline on motility, hyperactivity, and acrosome reaction of spermatozoa in rams. Veterinary Medicine and Public Health Journal Vol. 1, no. 3 (Sep. 2020), pp.81-84.
https://search.emarefa.net/detail/BIM-1426843

American Medical Association (AMA)

Atiyyah, Khalid Hafiz al-Shahhat Muhammad& Taysir, M. I.& Badr, M. R.& Zaki, K.. Investigating the effects of theophylline on motility, hyperactivity, and acrosome reaction of spermatozoa in rams. Veterinary Medicine and Public Health Journal. 2020. Vol. 1, no. 3, pp.81-84.
https://search.emarefa.net/detail/BIM-1426843

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references : p. 84

Record ID

BIM-1426843