Structural and functional effects of single nucleotide polymorphism on canine cytochrome b 5 reductase - in silico
Author
Source
Veterinary Medicine and Public Health Journal
Issue
Vol. 2, Issue 3 (30 Sep. 2021), pp.73-76, 4 p.
Publisher
Refaad Center for Studies and Research
Publication Date
2021-09-30
Country of Publication
Jordan
No. of Pages
4
Main Subjects
Abstract EN
The objectives of this study were to investigate the effects of single nucleotide polymorphism in Canine cytochrome b5 reductase using computational methods.
Data was obtained from database of National Centre for Biotechnology Information (db SNP) and computational software was used for the analysis.
The 3D protein structure was predicted using phyre 2 server.
PANTHER analysis predicted the effect of single nucleotide polymorphism (substitution of Isoleucine for Leucine at position 194) as damaging.
Analysis using the Mutpred 2 web application also indicated deleterious effects of the amino acid substitution.
Molecular mechanisms of structural changes in the amino acid were determined using Mutpred 2 to be altered ordered interface, gain of allosteric sites and altered metal binding.
The study indicated that the substitution of Isoleucine by Leucine at position 194 of the amino acid sequence (Ile 194 Leu) resulted in the destabilization of the amino acid structure leading to functional deviation in canine cytochrome b5 reductase.
American Psychological Association (APA)
Musa, S. Ibrahim. 2021. Structural and functional effects of single nucleotide polymorphism on canine cytochrome b 5 reductase - in silico. Veterinary Medicine and Public Health Journal،Vol. 2, no. 3, pp.73-76.
https://search.emarefa.net/detail/BIM-1428445
Modern Language Association (MLA)
Musa, S. Ibrahim. Structural and functional effects of single nucleotide polymorphism on canine cytochrome b 5 reductase - in silico. Veterinary Medicine and Public Health Journal Vol. 2, no. 3 (Sep. 2021), pp.73-76.
https://search.emarefa.net/detail/BIM-1428445
American Medical Association (AMA)
Musa, S. Ibrahim. Structural and functional effects of single nucleotide polymorphism on canine cytochrome b 5 reductase - in silico. Veterinary Medicine and Public Health Journal. 2021. Vol. 2, no. 3, pp.73-76.
https://search.emarefa.net/detail/BIM-1428445
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 75-76
Record ID
BIM-1428445