Geno-and neurotoxicity of dietary acrylamide to rats and its impacts on some serum biomarkers

Other Title(s)

السمية الجينية و العصبية الناتجة عن تعرض جرذان التجارب للأكريلامايد الغذائي و تأثير ذلك على بعض المؤشرات الحيوية في مصل الدم

Dissertant

Muslih, Shahd Z.

Thesis advisor

Suwaylih, Khalid

University

Birzeit University

Faculty

Faculty of Science

Department

Department Nutrition and Dietetics

University Country

Palestine (West Bank)

Degree

Master

Degree Date

2022

English Abstract

Acrylamide is a chemical compound with a potential cause of many toxic and carcinogenic effects.

The presence of acrylamide was reported in starch-rich foods baked or fried on hightemperature.

Studies on acrylamide levels in foodstuff marketed in Palestine are lacking, although imported and local food stuff, especially those exposed to high temperatures during production, may contain significant levels of acrylamide that can cause toxicity & carcinogenicity.

This study was planned to evaluate levels of acrylamide in foodstuff marketed in Palestine and to evaluate possible effects of acrylamide exposure on rats.

Therefore, a total of 48 rats were equally divided into 6 groups exposed orally to different treatments and doses of AA, OO and a control.

The groups were: Control, 10 mg/ml/kg AA, 30 mg/ml/kg AA, 60 mg/ml/kg AA, 1.5 ml/kg OO, and 1.5 ml/kg OO+ 60 mg/ml/kg AA.

Each rat was given acrylamide dose orally 5 days a week for 5 weeks One group was administered AA and olive oil to test for possible amelioration of negative impacts of AA.

Animal weights, food consumption and any behavioural symptoms of neurotoxicity were monitored and recorded throughout the experiment.

Besides, some serum biochemical tests (ALT, AST, glucose, & insulin) and genotoxicity tests using RAPD method were performed.

A total of 105 food stuff samples were purchased from the local market in Ramallah, Palestine to be analysed for the acrylamide content.

Acrylamide was extracted by different methods but no results were obtained from this part of the experiment as the recovery percentage of acrylamide was always below the detection limit of the LC-MS device used.

The effect of acrylamide on rats’ body weight starts to appear after four weeks of exposure.

The mean weight of the rats exposed to 60 mg/ml/kg AA was significantly less than the weight of the control rats (237.25 ± 12.75 g and 292.88 ± 8.28 g respectively).

On the other hand, weights of rats exposed to 60 mg AA were negatively affected as their mean body weight from the fourth week on was significantly less than the mean body weight of the control rats.

Food consumption of rats in each group was calculated as grams of consumed food per rat.

Results obtained showed that rats that were exposed to 1.5 ml OO+60 mg AA consumed significantly less food than control groups from the first week of the experiment to the end (fifth week).

In addition, food consumption of rats given 1.5 ml OO+60 mg AA was significantly less than the consumption by the rats that were given 1.5 ml OO only.

Acrylamide caused progressive gait abnormalities after four weeks of exposure to rats that were exposed to 30 mg/ml/kg AA and 60 mg/ml/kg AA.

Rats that were exposed to 30 mg/ml/kg AA have developed walking abnormalities and external rotation of the hind-limbs while the hindlimbs of rats that were exposed to 60 mg/ml/kg AA have totally paralyzed.

However, rats that were given 1.5 ml OO with 60 mg AA did not show any neurotoxicity symptoms.

RAPD profiles generated from DNA obtained before exposure and after exposure to AA revealed the formation of a total of 38 polymorphic bands representing around 11.5% of the total bands obtained after exposure.

All groups, except the control, generated polymorphic bands that ranged between 3 in the rats exposed to 10 mg/ml/kg AA and 15 in rats exposed to 30 mg/ml/kg AA.

These results indicate the genotoxicity of acrylamide.

ALT and AST activities were measured for the indication of liver damage.

Their activities were increased with the increase in acrylamide dose but the increase was not statistically significant.

Concentrations of insulin and glucose in serum were calculated for the indication of acrylamide effect.

There was no significant effect of acrylamide on either insulin nor glucose concentrations observed in this study.

At the end of the experiment, it was concluded that acrylamide affected the ability of the rats to consume food and gain weight.

Acrylamide caused neurotoxicity in rats as symptoms ranged from walking abnormalities to total paralysis in the hind-limbs of rats exposed to high dose of acrylamide.

RAPD analysis revealed that all doses of acrylamide caused genotoxicity to rats.

Levels of ALT and AST seemed to increase due to acrylamide exposure.

However, levels of insulin and glucose did not exhibit any significant change in response to acrylamide exposure.

Giving olive oil along with acrylamide (60 mg) protected against neurotoxicity.

Although this treatment did not improve neither body weight nor food consumption nor the genotoxic effect of acrylamide.

Main Subjects

Biology

No. of Pages

58

Table of Contents

• APA
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Language

English

Data Type

Arab Theses

Record ID

BIM-1429295