Determination of atenolol and hydrochlorothiazide in pharmaceutical preparation using RP-HPLC
Other Title(s)
تقدير اتينولول و هيدروكلوروثيازيد في المستحضر الصيدلاني باستعمال الطور العكسي لكروماتوغرافيا السائل عالي الأداء
Joint Authors
Najm, Leon
Antakli, Sad
Jumah, Mustafa al-Abu
Source
Journal of Natural Sciences, Life and Applied Sciences
Issue
Vol. 6, Issue 3 (30 Sep. 2022), pp.144-152, 9 p.
Publisher
Publication Date
2022-09-30
Country of Publication
Palestine (Gaza Strip)
No. of Pages
9
Main Subjects
Abstract EN
A (reversed phase high performance liquid chromatography) (RP-HPLC) method was development for the determination of atenolol (ATE) and hydrochlorothiazide (HCT) in tablets formulations.
isocratic elution chromatography analysis was achieved with (methanol : phosphoric acid) (pH = 4) in ratio of 70:30 (v/v) as mobile phase, the analytical is done by C18 column and UV detector at 220 nm, pump flow rate was 1.2 mL/min and sample injection volume 20 μL.
retention time was less than 3 min.
the method was validated with respect to (linearity, accuracy, specificity, precise, LOD and LOQ).
linearity showed a good correlation coefficients R2 = 0.9993 and R2 = 0.9997 for (ATE) and (HCT), respectively ranges were (20 – 100) ?g/mL for (ATE) and (3 – 15) ?g/mL for (HCT).
(The limit of detection) (LOD) and (limit of quantification) (LOQ) were to be 2.60 ?g/mL and 7.87 ?g/mL for (ATE), 0.25 ?g/mL and 0.77 ?g/mL for (HCT), respectively.
the proposed method was successfully applied to analysis individual or mixture of (ATE) and (HCT) in Syrian trademark drugs.
all studied samples showed that the drug levels were conformed to British pharmacopeia.
finally, the method is simple, selective and suitable for routine quality control analysis.
American Psychological Association (APA)
Antakli, Sad& Najm, Leon& Jumah, Mustafa al-Abu. 2022. Determination of atenolol and hydrochlorothiazide in pharmaceutical preparation using RP-HPLC. Journal of Natural Sciences, Life and Applied Sciences،Vol. 6, no. 3, pp.144-152.
https://search.emarefa.net/detail/BIM-1429408
Modern Language Association (MLA)
Antakli, Sad…[et al.]. Determination of atenolol and hydrochlorothiazide in pharmaceutical preparation using RP-HPLC. Journal of Natural Sciences, Life and Applied Sciences Vol. 6, no. 3 (Sep. 2022), pp.144-152.
https://search.emarefa.net/detail/BIM-1429408
American Medical Association (AMA)
Antakli, Sad& Najm, Leon& Jumah, Mustafa al-Abu. Determination of atenolol and hydrochlorothiazide in pharmaceutical preparation using RP-HPLC. Journal of Natural Sciences, Life and Applied Sciences. 2022. Vol. 6, no. 3, pp.144-152.
https://search.emarefa.net/detail/BIM-1429408
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 151-152
Record ID
BIM-1429408