Assessment of selective auto antibodies in pregnant women with spontaneous recurrent miscarriage and non-miscarriage in al-Gezira state-Sudan

Abstract EN

Recurrent miscarriage is a critical problem in the health sector.

Antiphospholipid, anticardiolipin, antinuclear and antidouble strand DNA are group of autoantibodies that bind to negatively charged phospholipids which has relation with recurrent miscarriage during the first 10 weeks of pregnancy or the birth of a dead child (still birth).

The aim of this study was to detect the presence of these antibodies among pregnant women in Al-Gezira state.

A case-controls study was conducted at Wad Madani Teaching Hospital Al- Gezira, Department of Obstetrics gynecological in Sudan.

The cases were (90 women) 45 women with miscarriage while 45 non-miscarriage women chosen as control.

Antiphospholipid, Anticardiolipin, Antinuclear and Antidouble strand DNA for IgG antibodies were analyzed in the maternal sera of all of the participants by using ELISA.

Furthermore all positive and borderline results were confirmed by using immunoblot test.

Out of 90 women, 45 women with miscarriage showed 2(4.4%) were positive, 2(4.4%) were negative for IgG APA 1(2.2%), 2(4.4%) were positive and borderline ACL IgG, 3(6.7%), 1(2.2%) were positive and borderline ANA IgG and negative in dsDNA while 45 women with non-miscarriage 1(2.2%) was borderline APL IgG, 1(2.2%)was borderline ANA IgG and negative in ACL and dsDNA .

There were significant difference in the age, biomass index, MCV, MCHC, MPV, RDWCV, RDWSD between the two groups (P < 0.05) .The analysis of In binary logistic regression women with variable, univariate and multivariate analysis showed that preeclampsia, vaginal diseases, vaginal bleeding, menstruation cycle, RDWC, RDWSD and biomass index were significantly associated with miscarriage in both (P < 0.05).

While age were significant associated with miscarriage in univariate analysis (OR= 5, 95%Cl=2-13, P value 0.001), family history (OR= 2.94, 95%Cl=2.946-948, P value.000) and antiphospholipid antibodies associated with miscarriage in multivariate miscarriage (OR= 4.4, 95%Cl=45-43.8, P value0.02).

In the current study antiphospholipid, anticardiolipin and antinuclear IgG sero-positivity are associated with miscarriage.