علاقة التعدد الشكلي-799TC لمعزاز جين MMP-8 بالتهاب النسج حول السنية لدى مرضى سوريين سكريين من النمط الثاني

Abstract EN

Introduction: The goal of the present study is to investigate the association between (-799C/T) polymorphism in MMP-8 gene promoter and chronic periodontitis (ChP) in Syrian patients with type II diabetes mellitus (DM).

Materials and Methods: A total of 180 participants were recruited in the study, which included 40 ChP, 40 DM, 60 ChP with DM (ChPDM) and 40 healthy controls.

Genomic DNA was extracted from the whole blood collected from the participants.

DNA was amplified using specific primers flanking -799C/T point in MMP-8 gene promoter.

The genotyping was performed by restriction fragment length polymorphism (RFLP) using SfcI enzyme.

The distribution of genotypes and allele frequencies between the study groups was compared using the Chi-square test.

Results: The genotype frequencies showed significant differences between each patient group and healthy group (P< 0.05), and subjects carrying the T allele were significantly 2.5 times more likely to develop ChP with DM (OR= 2.513, CI:1.373 to 4.598, P = 0.0018) and without DM (OR= 2.645, CI:1.420 to 4.923, P = 0.0026) compared with C subjects.

Conclusion: The present study suggests that the MMP8 (-799C/T) polymorphisms has an important role in periodontitis susceptibility in Syrian diabetic population.