Establishment somatic embryogenesis of some mango rootstocks in vitro

Joint Authors

Abd al-Aziz, Husni Fathi
Hamdi, A. E.

Source

Annals of Agricultural Science, Moshtohor

Publisher

Banha University Faculty of Agriculture

Publication Date

2021-12-31

Country of Publication

Egypt

No. of Pages

8

Main Subjects

Agriculture

English Abstract

This work aimed to develop a conservation protocol for date palm cv.

Amry, an Egyptian semi dry rare cultivar.

Slow growth conditions of storage studied by using low temperature at 15°C, beside the manipulation of salt strength of nutrient medium,( MS, 1/2 MS or1/4 MS) in combination with osmotic agent addition sucrose, sorbitol, or mannitol at constant concentration (0.3 M) for each, to conserve somatic embryos clusters explants, which obtained previously through direct somatic embryogenesis of inflorescences explants.

Survival percentage and recovery were evaluated after 6 months of conservation period.

Results showed that conserved somatic embryos clusters explants on full MS, ½ MS, conservation medium supplemented with sucrose or sorbitol recorded the highest significant survival percentage on full MS, ½ MS, where the lowest significant survival percentage was obtained when somatic embryos were conserved on 1/4 medium supplemented with mannitol .

Best shoot number and shoot length recovery under normal growth conditions achieved on conservation medium supplemented with sucrose in comp inaction with reducing the full MS salt strength to the half for 6 months of conservation period under low temperature at 15°C.

Data Type

Conference Papers

Record ID

BIM-1432393

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