Kinetic studies of catalase and peroxidase enzymes extracted from garlic cloves (Allium sativum L.)
Other Title(s)
دراسات حركيات إنزيمات الكاتاليز و البيروكسيديز المستخلصة من فصوص الثوم
Joint Authors
Fudah, Farahat Fudah Ali
Sad, Salah Mustafa
al-Hadari, Abd Allah al-Sayyid
al-Khayyat, Najwa Ibrahim
Source
Annals of Agricultural Science, Moshtohor
Publisher
Banha University Faculty of Agriculture
Publication Date
2021-12-31
Country of Publication
Egypt
No. of Pages
8
Main Subjects
English Abstract
Garlic cloves belongs to the genus Allium and family liliaceae, is one of the more commonly used health supplements and its therapeutic benefits.
Many attempts were carried out to elucidate its importance since it contains natural antioxidant enzymes, i.e.
catalase (CAT) and peroxidase (POD).
These natural enzymes were extracted from garlic cloves and their activities and kinetic characterization were investigated.
The results indicated that the activity, protein content and specific activity of catalase and peroxidase were (2.05 Units/ml, 204.4 Units/ml), (4.2 mg/ml, 5.11mg/ml) and (0.448 Units/mg protein, 40 Units/mg protein), respectively.
The optimum pH and temperature of these enzymes under investigation were 7.0, 5.5 and 40ºC, 50ºC, respectively.
The Km and Vmax for catalase and peroxidase enzymes were equalled to (1.88 ml/100ml, 0.37ml/100ml) and (6.43 Units/ml/min, 16.58Units/ml/min) , respectively.
Data Type
Conference Papers
Record ID
BIM-1432398
American Psychological Association (APA)
al-Khayyat, Najwa Ibrahim& Sad, Salah Mustafa& Fudah, Farahat Fudah Ali& al-Hadari, Abd Allah al-Sayyid. 2021-12-31. Kinetic studies of catalase and peroxidase enzymes extracted from garlic cloves (Allium sativum L.). . Vol. 59, Special issue (2021), pp.331-338.Qalyoubia Egypt : Banha University Faculty of Agriculture.
https://search.emarefa.net/detail/BIM-1432398
Modern Language Association (MLA)
al-Khayyat, Najwa Ibrahim…[et al.]. Kinetic studies of catalase and peroxidase enzymes extracted from garlic cloves (Allium sativum L.). . Qalyoubia Egypt : Banha University Faculty of Agriculture. 2021-12-31.
https://search.emarefa.net/detail/BIM-1432398
American Medical Association (AMA)
al-Khayyat, Najwa Ibrahim& Sad, Salah Mustafa& Fudah, Farahat Fudah Ali& al-Hadari, Abd Allah al-Sayyid. Kinetic studies of catalase and peroxidase enzymes extracted from garlic cloves (Allium sativum L.). .
https://search.emarefa.net/detail/BIM-1432398
