Comparative analysis of cytokines and growth factors in the conditioned media of stem cells from the pulp of deciduous, young, and old permanent tooth

Joint Authors

Testarelli, Luca
Baashin, Husam Ali
Vyas, Nishant
Bhandi, Shilpa
al-Khatani, Ahmad
Sumayli, Hasan Abd al-Aziz
Sabyei, Mushyirah Yahya
al-Zaylai, Abd al-Aziz Muhammad
Sumayli, Muhammad Ali
Hakmi, Hanan Ibrahim
Jafar, Muhammad Abd al-Rubb
Patil, Shankargouda

Source

Saudi Journal of Biological Sciences

Issue

Vol. 28, Issue 6 (30 Jun. 2021), pp.3559-3565, 7 p.

Publisher

Saudi Biological Society

Publication Date

2021-06-30

Country of Publication

Saudi Arabia

No. of Pages

7

Main Subjects

Dental

Abstract EN

tooth (SHEDs), young (yDPSCs), and old permanent tooth (oDPSCs).

Methods : All the stem cells were assessed for mesenchymal stem cell markers.

the stem cells were differentiated into osteoblasts and chondrocytes using lineage-specific differentiation media.

Conditioned media was collected from growing stem cells, and a cytometric bead array was performed to estimate secreted cytokines and growth factor levels by flow cytometry.

gene expresseion levels were assessed by real-time quantitative polymerase chain reaction.

results : age did not affect the mesenchymal characteristics of dental stem cells from various age groups.

The secretomes of SHEDs and young yDPSCs exhibit more growth factors and lesser pro-inflammatory cytokines than oDPSCs.

Osteo and chondrogenic differentiation potential were higher in SHEDs and young yDPSCs than in the oDPSCs.

TLR1, TLR2, TLR3 show decreased expression levels with age and TLR5, TLR6 show increased expression with age.

Conclusion : the superior regenerative potential of SHEDs and yDPSCs may be due to the higher growth factors and lower pro-inflammatory cytokine profile.

American Psychological Association (APA)

Bhandi, Shilpa& al-Khatani, Ahmad& Sumayli, Hasan Abd al-Aziz& Sabyei, Mushyirah Yahya& al-Zaylai, Abd al-Aziz Muhammad& Sumayli, Muhammad Ali…[et al.]. 2021. Comparative analysis of cytokines and growth factors in the conditioned media of stem cells from the pulp of deciduous, young, and old permanent tooth. Saudi Journal of Biological Sciences،Vol. 28, no. 6, pp.3559-3565.
https://search.emarefa.net/detail/BIM-1436493

Modern Language Association (MLA)

Bhandi, Shilpa…[et al.]. Comparative analysis of cytokines and growth factors in the conditioned media of stem cells from the pulp of deciduous, young, and old permanent tooth. Saudi Journal of Biological Sciences Vol. 28, no. 6 (2021), pp.3559-3565.
https://search.emarefa.net/detail/BIM-1436493

American Medical Association (AMA)

Bhandi, Shilpa& al-Khatani, Ahmad& Sumayli, Hasan Abd al-Aziz& Sabyei, Mushyirah Yahya& al-Zaylai, Abd al-Aziz Muhammad& Sumayli, Muhammad Ali…[et al.]. Comparative analysis of cytokines and growth factors in the conditioned media of stem cells from the pulp of deciduous, young, and old permanent tooth. Saudi Journal of Biological Sciences. 2021. Vol. 28, no. 6, pp.3559-3565.
https://search.emarefa.net/detail/BIM-1436493

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references : p. 3564-3565

Record ID

BIM-1436493