Effects of cyclosporine therapy on liver and kidney retrieval in poisoned male rats by Mesobuthus eupeus scorpion venom

Abstract EN

Background:MesobuthuseupeusvenomisamemberofButhidaefamily,whichcanenterthebloodcirculationexertingdetrimental effectsonbodyorgans,suchastheliverandkidneythroughinflammation.

Cyclosporine,knownasananti-inflammatorydrug,is usedtotreatmanyinflammation-associateddiseases.

Objectives: Inthisstudy,cyclosporinewasselectedtoinhibitthescorpiontoxineffectsonratorgans.

Methods: Thisexperimental studywas conductedinthe RaziVaccine andSerum ResearchInstitute, AgriculturalResearch EducationandExtensionOrganization,Karaj,Iran,fromJunetoNovember2019.Fiftymaleratswererandomlydividedintofivegroupsof 10,includingthecontrol(10mg/kgoliveoili.p),M.eupeusvenom(10mg/kgi.p.),cyclosporine10mg/kg(venom10mg/kgfor30min i.pfollowedbycyclosporine10/kgmgfor7dayi.p.),cyclosporine20mg/kg(venom10mg/kgfor30mini.pfollowedbycyclosporine 20mg/kgfor7dayi.p.),andcyclosporine30mg/kg(venom10mg/kgfor30mini.pfollowedbycyclosporine30mg/kgfor7dayi.p.).

Aftertreatmentwithcyclosporine,theliverandkidneyfunctionwasanalyzedbycalculatingsomebiochemicalenzymes,including serumglutamate-pyruvatetransaminase(SGPT),serumglutamicoxaloacetictransaminase(SGOT),nitricoxide(NO),interleukin-2 (IL-2), malondialdehyde(MDA), creatinine, andurea viaELISAand spectrophotometry.

Then, to determinethe rateof apoptosis in tissue,terminaldeoxynucleotidyltransferase-mediateddUTPnick-endlabelingmethodwasdone.

Results:Attheendof thestudy,theresultsshowedasignificantelevationinSGPT(164.5±10vs.126.2±7,P< 0.0001),SGOT(190.37 ±11vs.

148±10,P< 0.0001),NO(24.4±1.17vs.

17.4±1.4,P=0.02),andMDA(0.42±0.05vs.

0.22±0.04,P< 0.0001)inthevenom groupcomparedwiththecontrolgroup.

Therewerenosignificantdifferencesintheurea,IL-2,andcreatininebetweenthevenom andcontrolgroups.However,thegroupreceivingcyclosporine(30mg/kg)showedasignificantdeclineinSGPT(96.42±5.7vs.164.5 ±10, P < 0.0001), SGOT (144.57±9.24 vs.

190.37±11, P < 0.0001), urea (28.83±1.32 vs.

38.83±1.6, P = 0.00), creatinine (0.023± 0.01vs.

0.29±0.005,P< 0.0001),andMDA(0.10±0.01vs.

0.42±0.05,P< 0.0001),aswellasincreasedapoptosisrate(P< 0.05), compared with the venom group.

No significant difference was observed between the cyclosporine and venom groups in NO and IL-2.

Conclusions: Cyclosporineatadoseof 30mgwasabletodecreaseinflammatoryresponsesandinduceapoptosisrate.

Therefore, itcouldbeasuitabledrugforpatientsbittenbyascorpionsting.