
Can the irrigation of soils with Amoxicillin-enriched water cause the proliferation of Bacteria resistant to antibiotics among culturable heterotrophic aerobic soil bacteria?
Other Title(s)
هل يمكن أن يؤدي ري التربة بالمياه الغنية بالمضاد الحيوي ألأموكسيسيلين (Amoxicillin) إلى انتشار البكتيريا المقاومة للأموكسيسيلين بين بكتيريا التربة الهوائية غير ذاتية التغذية (البكتيريا العضوية) ؟
Joint Authors
Blackburn, Daniel
al-Kalbani, Adhari
al-Sayyabi, Buthaynah
al-Kasabi, Muhammad
al-Busaidī, Ahmad
Source
Agricultural and Marine Sciences : Research Journal
Issue
Vol. 27, Issue 2 (31 Dec. 2022), pp.10-18, 9 p.
Publisher
Sultan Qaboos University College of Agricultural and Marine Sciences
Publication Date
2022-12-31
Country of Publication
Oman
No. of Pages
9
Main Subjects
Earth Sciences, Water and Environment
Abstract EN
This study investigated the short-term impact of irrigation with Amoxicillin solutions on the presence of the amoxicillin-resistance trait among culturable soil heterotrophic aerobic bacteria.
The microcosm experimental design consisted of 15 days of incubation of 10 g soil samples irrigated daily with distilled water containing increasing doses of amoxicillin (0, 0.1, 1, 10, 100, 1000 µg g-1 of soil day-1).
The hypothesis was that continuous daily addition of antibiotics would increase the proportion of antibiotic-resistant bacteria in soils.
After the incubation period, the total and antibiotic resistance heterotrophic aerobic bacteria communities were assessed through serial dilution of soil suspensions, followed by agar plate culture enumeration, isolation, identification and microscopy observation.
The presence of antibiotic-resistant bacteria was also evaluated directly on treated wastewater used for field irrigation before this microcosm study to assess the amoxicillin-resistant bacteria bioaugmentation hypothesis.
Results indicated that the Amoxicillin resistance was widespread among bacteria present in both treated wastewater used for irrigation and in the receiving soil.
A microcosm experiment was attempted as a ‘proof of concept’ to demonstrate that irrigation with treated wastewater containing antibiotics would exert selective pressure and promote the proliferation of antibiotic resistance bacteria.
Unexpectedly, the results from the microcosm incubations indicated the daily addition of amoxicillin did not increase bacterial antibiotic resistance trait abundance in soils, which even significantly decreased for all tested doses.
The antibiotic-resistant species identified among the isolates were Pseudomonas mosselii, P.
otitidis, P.
mendocina, P.
flavescens, Stenotrophomnas maltophilia, Bacillus thuringiensis, Aeromonas veronii, Candida parapsilosis, Streptomyces violaceoruber and Microbacterium barkeri.
American Psychological Association (APA)
al-Kalbani, Adhari& Blackburn, Daniel& al-Sayyabi, Buthaynah& al-Kasabi, Muhammad& al-Busaidī, Ahmad. 2022. Can the irrigation of soils with Amoxicillin-enriched water cause the proliferation of Bacteria resistant to antibiotics among culturable heterotrophic aerobic soil bacteria?. Agricultural and Marine Sciences : Research Journal،Vol. 27, no. 2, pp.10-18.
https://search.emarefa.net/detail/BIM-1439663
Modern Language Association (MLA)
al-Kalbani, Adhari…[et al.]. Can the irrigation of soils with Amoxicillin-enriched water cause the proliferation of Bacteria resistant to antibiotics among culturable heterotrophic aerobic soil bacteria?. Agricultural and Marine Sciences : Research Journal Vol. 27, no. 2 (2022), pp.10-18.
https://search.emarefa.net/detail/BIM-1439663
American Medical Association (AMA)
al-Kalbani, Adhari& Blackburn, Daniel& al-Sayyabi, Buthaynah& al-Kasabi, Muhammad& al-Busaidī, Ahmad. Can the irrigation of soils with Amoxicillin-enriched water cause the proliferation of Bacteria resistant to antibiotics among culturable heterotrophic aerobic soil bacteria?. Agricultural and Marine Sciences : Research Journal. 2022. Vol. 27, no. 2, pp.10-18.
https://search.emarefa.net/detail/BIM-1439663
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 17-18
Record ID
BIM-1439663