Evaluation of multi-locus sequence analysis of different genes in rhizobium-legume incompatibility
Joint Authors
Idris, Abd al-Malak Umar Ahmad
Sulayman, Abd al-Munim al-Hadi
Source
Sudan Journal of Science and Technology
Issue
Vol. 21, Issue 1 (30 Jun. 2020), pp.38-46, 9 p.
Publisher
Sudan University of Science and Technology Deanship of Scientific Research
Publication Date
2020-06-30
Country of Publication
Sudan
No. of Pages
9
Main Subjects
Abstract EN
This study was performed to characterize TAL169 bacteria which is used widely as bio-inoculants through sequencing of 16SrRNA, nifH, nodC, recA, and glnII genes.
DNA of the bacteria was extracted, PCR amplification of different genes and sequencing were done.
The results indicated that TAL169 is Mesorhizobium with 99% identity according to 16SrRNA, recA and glnII genes sequences analysis.
At the same time, nifH gene analysis indicated that it is Sinorhizobium with the same identity.
The phylogeny analysis of all genes placed TAL169 bacterium in separate categories.
The study concluded that there is no genetic relationship between TAL169 and Bradyrhizobium which used in inoculation trials in different regions in the world and was named as TAL169.
That is why the majority of previous studies findings used this bacterium as Baradyrhizobium in bio-inoculation recorded no significant differences in the production of legumes inoculated.
So using of this bacterium is a useless process.
Accordingly, for successful bio-inoculation and production improvement in agriculture, before preparation of the bacterial inoculants identification of locally isolated Rhizobia by sequencing and analyzing of the above-mentioned genes or more housekeeping genes is recommended.
American Psychological Association (APA)
Idris, Abd al-Malak Umar Ahmad& Sulayman, Abd al-Munim al-Hadi. 2020. Evaluation of multi-locus sequence analysis of different genes in rhizobium-legume incompatibility. Sudan Journal of Science and Technology،Vol. 21, no. 1, pp.38-46.
https://search.emarefa.net/detail/BIM-1442752
Modern Language Association (MLA)
Idris, Abd al-Malak Umar Ahmad& Sulayman, Abd al-Munim al-Hadi. Evaluation of multi-locus sequence analysis of different genes in rhizobium-legume incompatibility. Sudan Journal of Science and Technology Vol. 21, no. 1 (Jun. 2020), pp.38-46.
https://search.emarefa.net/detail/BIM-1442752
American Medical Association (AMA)
Idris, Abd al-Malak Umar Ahmad& Sulayman, Abd al-Munim al-Hadi. Evaluation of multi-locus sequence analysis of different genes in rhizobium-legume incompatibility. Sudan Journal of Science and Technology. 2020. Vol. 21, no. 1, pp.38-46.
https://search.emarefa.net/detail/BIM-1442752
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 44-46
Record ID
BIM-1442752