Impact of bacillus subtilis, trichoderma SPP. and the bioproduct top perfect on meloidogyne incognita infecting pepper plants

Abstract EN

Root-knot nematodes are considered one of the severe plant pathogens that can be managed effectively with chemical nematicides in spite of their hazards to humans and the environment.

our work focus on the efficacy of bacillus subtilis, trichoderma spp.

culture filtrates, the bioproduct top perfect and the nematicide Vydate ® L 24% for controlling meloidogyne incognita infecting pepper plants Cv.

balady.

under laboratory and greenhouse conditions, treatments with 0.025 and 0.05 μl of Vydate ® L 24%/ml distilled water caused the highest reductions ranging from 83.9-99% in nematode egg-hatching and increased 2nd stage juveniles’ mortality; the number of root galls and egg masses, followed by treatments with the (100 μl / ml, 10 ml/200 cc soil and 50 ml / kg soil) of B.

subtilis, trichoderma culture filtrates, and the bioproduct top perfect which showed 60.4-79.8% inhibition.

meanwhile, treatments with the low doses (50 μl / ml, 5 ml / 200 cc soil and 25 ml / kg soil) of b.

subtilis, trichoderma culture filtrates and the bioproduct, top perfect resulted in 23.5-59.6% inhibition in nematode parameters compared with the check treatment.

in comparison to check treatment under greenhouse conditions application of Vydate ® L 24% showed a 60.2-77.8% increase in growth parameters of pepper plants, followed by both treatments with high and low doses of b.

subtilis, trichoderma culture filtrates, and the bioproduct top perfect which showed 43.1-58.8% and 29.7-42.0% increase, respectively.

tested biocontrol agents could achieve various degrees of Root-knot nematode control on pepper plants infected with M.

incognita under laboratory and greenhouse conditions.