Genetic diagnosis of root nodules bacteria isolated from some leguminous plants in Nineveh governorate

Abstract EN

Because of the importance of atmospheric nitrogen-fixing bacteria, the study aimed to isolate bacteria from the root nodules of plants from four areas within the city of Mosul during the winter planting period, for the season 2020-2021.

Biochemical tests, phenotypic and agricultural characteristics were used to diagnose bacterial isolates and study their sensitivity and resistance to ten types of antibiotics.

The results showed that the highest percentage of resistance was to the antibiotics Trimethoprim and Streptomycin at 100%, while it was the least resistant to the antibiotics Tetracycline and Rifampicin at 22.2%.

The four bacterial isolates were resistant to cadmium chloride salts (CdCl), while the lowest growth rate was when treated with mercury chloride salt (HgCl).

Polymerase Chain Reaction (PCR) technology was used to diagnose samples based on the analysis of nitrogenous bases in the 16S rRNA gene and compare the sequences generated by DNA amplification with standard isolates within NCBI to detect new isolated bacterial isolates.