Molecular study of Acinetobacter baumannii using 16s rrna and bla Oxa-51 Gene Isolated from Hospitals in Duhok-kurdistan Region, Iraq

Abstract EN

Background and objective: Acinetobacter baumannii is pathogenic and multiresistant bacteria that cause nosocomial infection.

The aim of this study are diagnosis A.

baumannii by using specific bla OXA-51 and 16SrRNA.

Methods: out of 150 Acinetobacter species samples were collected from patients in Azadi teaching hospital, Duhok Emergency hospital; and burn Duhok cosmetic and burn hospital from May 2020 to August 2020.

The samples were phenotypically diagnosed by bacteriological strategy.

Out of 100 samples were selected and by molecular level confirmed as Acinetobacter spp.

Results: isolates were revealed as small, pale, and late-lactose fermenter colonies on MacConkey agar appeared as a creamy, opaque, andnon-hemolytic colony on blood agar All suspected isolates, as A.

baumannii, were growing at 44ºC.

By using the genus-specific bla OXA-51 primer that produced 353 bp amplification band and 75 samples were diagnosed by PCR as A.

baumannii by 16S rRNA as a specific primer and showed 150 bp amplicon.

10 samples out of the 75 resembling A.

baumannii were identified as a multidrug resistant isolates by method of diffusion.

Disc, A.

baumannii isolates displayed a high resistance rate of 85% to azithromycin and 80% to imipenem.

Moreover, amikacin, meropenem, and gentamycin, Trimethoprim-Sulfamethoxazole, and ciprofloxacin, norfloxacin showed a low level of efficacy against A.

baumannii isolates with the resistant rate of 88%, 90%, 90%, and 93% respectively