Molecular characterization of virulence factors among antibacterial resistant pseudomonas aerugınosa isolated from burn infections from Duhok and Erbil hospitalsIraq

Abstract EN

In the current study, 225 isolates of P.

aeruginosaburn infection have been collected from major hospitals in Duhok and Erbil / Iraq, from April 2015 till to September 2015.

One hundred thirty-six of these were isolated from males accounting 60.4 % whereas 89(39.6%) of them were recovered from females.

One hundred of these isolates were selected (fifty from each province including; Erbil and Duhok Province) and subjected to 16 different antibiotics using disc diffusion method showed high resistancy to most tested antibiotics, 90% of these isolates were multidrug resistant, Imipeneme was considered as the most effective antibiotic effect on these isolates with resistant rate 47%.The genome all of these isolates were successfully amplified producing a single band of the 16srDNA locus in all strains with a molecular weight of about 956 bp in order to confirm at molecular level that all these isolates were P.

aeruginosa.

The results of the detection of five virulence related genes including (opr-1, tox-A,exo-S,las-B, and nan-1) revealed that ten of these isolates accounting (10%) lacked any tested virulence markers,oprIas a marker for presence of pathogenicity island was the most predominant marker among all other virulence markers accounting 90(90%) followed by tox-A, and exo-S accounting 86(86%) and 86(86%) respectively, while the prevalence of las-Bgene is found with the rate 82(82%) and nan-1with35(35%)respectively.