Effect of intake supplementation choline powder and food rich of it on immune response and oxidative stress induced by diazinon toxicity in rats

Abstract EN

Diazinon is an organic compound of organophosphate insecticide formerly used to control cockroaches, silverfish, ants, and fleas in residential.

choline is one of the essential nutrients for the body, which has the characteristic of solubility in water, and it is usually composed of b-complex vitamins.

generally choline refers to different types of quaternary ammonium salts, it is important for maintaining good health in the body and strengthening the immune system.

this study aimed to verify the protective effects of food rich in choline against daizinon intoxication in rats infected with oxidative stress and on immune response.

forty male wistar rats weighing 170 ± 20g were divided into four equal groups ; 10 animals rats (negative group) and 30 rats were take diazinon in dissolved (70 mg / Kg bw orally daily for four weeks), which were classified into the control positive group and two treated rats groups that treated by choline powder (20 mg / kg orally daily for four weeks), peanuts (5, 5 g daily for four weeks, this average taken per rat) respectively.

rats were orally administered their relevant doses daily for four weeks.

blood samples were collected and picture (CBC) and serum was separated to evaluate the different immunological parameters and biochemical.

the results revealed that, all tread groups and the negative group showed a significant increase in blood levels of IFN ɤ, RBCs, Hb, Mcv, WBCs, lymphocytes, monocytes count, GPx ,GSH , SOD and CTA, but a decrease in IL-1β, TNF-α, MCV, AST, ALT, creatinine, urea and uric acid compared with a positive group.

while the fourth group (choline in nutrition) showed improvement than third group (choline powder) in IL-1β, TNF-α, Hb, Mcv, lymphocytes, monocytes, GPx, SOD,CTA, AST , alt and uric acid.

group 4 which intake food rich in choline revealed no histopathological changes in the liver and kidney.

in conclusion, taking foods rich in choline significantly reduces the acute immune response and oxidative stress so it can be replaced with nutritional supplements.