In vitro propagation of pyracantha coccinea as affected by growth regulators and different carbon sources

Abstract EN

The aim of this study was to investigate the effect of different types and concentration of plant growth regulators (cytokinins : kinetin and benzyl adenine (BA) at 0, 0.5, 1, 1.5, 2 and 2.5 mg.l* ¹) on shoot proliferation of pyracantha coccinea and auxin (indole-3- butyric acid) (IBA at 0, 0.5 and 1 mg.I-¹) and different carbon sources (Sucrose and Glucose) with ½ and full MS salt strength in root formation.

the results obtained from this study indicate that the greatest number shoots and leaves per explant were acquired when BA was 2.5 mg/1¹, which gave 8 shoots and 54.33 leaves respectively.

In the rooting stage, the greatest number of roots per explant was obtained when half MS salt strength was used with sucrose and Img.1¹ IBA, which gave 11.33 roots/explant.

Up to 83% of shoots were rooted.

plantlets of P.

coccinea with roots were successfully acclimatized with a survival rate of 80% in sand, 95% in peatmoss and 85% in sand + peatmoss.

this procedure can be adopted for P.

coccinea in vitro propagation.