تحديد المحتوى الكلي من الفينولات في نباتات دم الغزال Helichrysum Sanguineum L. و المصيص Phlomis Viscosa Poiret و زهرة الألماسة Paronychia Argentea Lam.

Abstract EN

Plants are an important source of many phenolic compounds due to antioxidant activity and role of this activity in many cases of treatment and prevention of cancer.

this research was conducted to determine total content of phenols and antioxidant activity of plants helichrysum sanguineum L; Phlomis viscosa Poiret and paronychia argentea lam.

which widespread in Syria, and to investigate their effect on viability of cancer cells, where compounds of plants were extracted using the soxhlet method with methanol.

the antioxidant activity of plant extracts was defined by determining their total phenol content, and their scaving of 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) radical.

the effect of these extracts was studied on viability of human hepatocellular carcinoma cells HepG2, compared to a stander drug by XTT reagent, with six concentrations per plant extract and for different treatment periods, 24, 48, 72 h.

the results showed that total phenolic content was higher in methanol extract of plant Helichrysum sanguineum, and there was no statistically significant difference in scaving effect of free radical DPPH for three plants.

the results of cell viability test also showed efficacy of plant extracts on HepG2 cells after 24h and 48h for extract of paronychia argentea, where IC50 reached 224.55 µg/mL and 14 µg/mL, respectively, and after 72h for the three plants at IC50 6.708 µg/mL for Paronychia argentea, 37.958 µg/mL for helichrysum sanguineum L.

and 53.650 µg/mL for phlomis viscosa.