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Determination of antimicrobial resistance profiles and molecular detection of carbapenemases in gram negative bacilli isolated from different sources in Mosul city, Iraq
Author
Source
Issue
Vol. 50, Issue 1 A (31 Jan. 2023), pp.1-15, 15 p.
Publisher
Kuwait University Academic Publication Council
Publication Date
2023-01-31
Country of Publication
Kuwait
No. of Pages
15
Main Subjects
Abstract EN
The objective of the present study was to evaluate the occurrence and dissemination of carbapenemases in Mosul city, Iraq.
Bacterial isolates were recovered from clinical, veterinary, and environmental specimens and samples.
Pure isolates were identified and tested for determination of their antimicrobial susceptibility profiles using disk diffusion method.
Phenotypic detection of metallo-carbapenemase-producing bacteria was conducted using combined disk method.
Imipenem-resistant strains were subjected to molecular detection of carbapenemase genes ( bla VIM , bla KPC , bla NDM , bla OXA-48 , bla IMP ) using multiplex PCR.
Three hundred and ninety three bacterial isolates were recovered from (365) specimens and samples, 246 isolates (62.6%) were multi-drug resistant (MDR) and 49 isolates (12.5%) were resistant to imipenem.
The study found that (7.4%) of the isolates were metallo-carbapenemase producers, phenotypically.
Multiplex PCR results revealed that 47/49 (95.5%) imipenem-resistant isolates were positive for PCR detection test, isolates with double genes ( bla KPC + bla VIM and bla KPC + bla NDM ) were the most prevalent (n=9; 18.4% for each), followed by bla KPC and bla NDM (n=8; 16.3% for each), and bla VIM (n=6; 12.2% ).
Three isolates (6.1%) were positive for bla IMP and two others (4.1%) were positive for each of bla OXA-48 and bla KPC + bla OXA-48 , while two isolates (4.1%) gave a negative result for the test.
In conclusion, carbapenemase genes were detected in the environmental isolates as well as in the clinical and veterinary ones, which might suggest the transmission of carbapenemase-producing bacteria and /or resistance determinants from clinical and veterinary settings to the environment.
American Psychological Association (APA)
al-Hasu, Mahmud Zaki. 2023. Determination of antimicrobial resistance profiles and molecular detection of carbapenemases in gram negative bacilli isolated from different sources in Mosul city, Iraq. Kuwait Journal of Science،Vol. 50, no. 1 A, pp.1-15.
https://search.emarefa.net/detail/BIM-1499936
Modern Language Association (MLA)
al-Hasu, Mahmud Zaki. Determination of antimicrobial resistance profiles and molecular detection of carbapenemases in gram negative bacilli isolated from different sources in Mosul city, Iraq. Kuwait Journal of Science Vol. 50, no. 1 A (Jan. 2023), pp.1-15.
https://search.emarefa.net/detail/BIM-1499936
American Medical Association (AMA)
al-Hasu, Mahmud Zaki. Determination of antimicrobial resistance profiles and molecular detection of carbapenemases in gram negative bacilli isolated from different sources in Mosul city, Iraq. Kuwait Journal of Science. 2023. Vol. 50, no. 1 A, pp.1-15.
https://search.emarefa.net/detail/BIM-1499936
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 11-15
Record ID
BIM-1499936