Biodegradation of tannins from polluted sources using natural enzymes

Abstract EN

This study aimed to characterize an efficient tannin-degrading microorganism through tannase production.

ten fungal isolates were screened on tannic acid medium.

the highest tannase producer was selected and identified as cryptococcus sp.

NRC10 with homology level 99.9%.

medium II at 45°C for 24 h at pH 5 were the most favourable conditions required for maximum enzyme production.

glucose and NH,CI were the best carbon and nitrogen sources, respectively.

the best conditions for the purified enzyme were 0.20% of tannic acid and 0.15 ml of tannase enzyme at 45°C after 10 minutes with pH 6.

the addition of Mn²+ stimulated the enzyme activity.

the purified enzyme was used for the degradation of tannins in red sea water and green tea leaves samples.

the amount of tannins after tannase addition was decreased.

in conclusion, tannase has a role in the removal of tannins in contaminated water and soil.