Optimization of hemolysin formation in Alcaligenes species isolated from abattoir wastewater samples in Akure, Ondo State, Nigeria

Abstract EN

Hemolysin is significantly toxic and is used as a molecular marker for pathogenicity.

This study evaluates the conditions for optimal hemolysin production by Alcaligenes faecalis strains isolated from a city abattoir wastewater.

The parameters investigated for hemolysin formation were the size of the inoculum, initial pH of the production medium, bacterial incubation temperature, agitation speed, and growth media.

After that, the effect of various parameters on the hemolytic activity for the formation of hemolysin was assessed.

The genus Alcaligenes was assigned to the test organisms after analyzing their 16S rRNA gene sequence with accession numbers: MF498824, MF498825, and MF498827.

Optimum conditions for hemolysin formation in Alcaligenes faecalis strain OS42 were inoculum size of 0.5% (v/v), pH 9, 20 o C, 0 rpm, and brain heart infusion broth.

77% and 79% of Hemolytic activities were achieved at 20 h for strains OS42 and OS61.

Cholesterol and ethylenediaminetetraacetic acid did not affect hemolysin formation.

This work revealed that the hemolysin formation in Alcaligenes strains was sourced from abattoir wastewater effluent.

The effluent was contaminated with pathogenic Alcaligenes strains, a public health hazard to their prospective infection of humans and animals.