In vitro propagation of Nanorrhinum ramosissimum (Wall.) Betsche: A traditionally important medicinal plant
Joint Authors
Sharma, Savita
Koul, Anuja
Shankarayan, Raju
Mallubhotla, Sharada
Sharma, Jyotsna
Source
Issue
Vol. 48, Issue 3 (31 Jul. 2021), pp.1-10, 10 p.
Publisher
Kuwait University Academic Publication Council
Publication Date
2021-07-31
Country of Publication
Kuwait
No. of Pages
10
Main Subjects
Abstract EN
An efficient micropropagation protocol facilitates successful conservation and improvement of Nanorrhinum ramosissimum (Wall.) Betsche by biotechnological means.
Shoot tip explants exhibited optimal organogenic response when inoculated on half-strength(1/2) Murashige and Skoog (MS) medium supplemented with kinetin (KN) and indole-3-acetic acid (IAA) (0.5 mg/L each).
Shoot organogenesis was further enhanced when the multiplication medium was fortified with dextrose (1%) (2.6 shoots/explant; 7.9 cm shoot length).
The regenerated shoots formed roots; however, the best rooting frequency (87%) was achieved on half-strength MS medium containing only IAA (0.5 mg/L).
Four-week-old in vitro plantlets were acclimatized with 95% survival under greenhouse conditions.
The regeneration protocol developed in this study can be utilized for germplasm conservation of this elite traditional medicinal plant.
American Psychological Association (APA)
Sharma, Jyotsna& Koul, Anuja& Sharma, Savita& Shankarayan, Raju& Mallubhotla, Sharada. 2021. In vitro propagation of Nanorrhinum ramosissimum (Wall.) Betsche: A traditionally important medicinal plant. Kuwait Journal of Science،Vol. 48, no. 3, pp.1-10.
https://search.emarefa.net/detail/BIM-1503288
Modern Language Association (MLA)
Sharma, Jyotsna…[et al.]. In vitro propagation of Nanorrhinum ramosissimum (Wall.) Betsche: A traditionally important medicinal plant. Kuwait Journal of Science Vol. 48, no. 3 (Jul. 2021), pp.1-10.
https://search.emarefa.net/detail/BIM-1503288
American Medical Association (AMA)
Sharma, Jyotsna& Koul, Anuja& Sharma, Savita& Shankarayan, Raju& Mallubhotla, Sharada. In vitro propagation of Nanorrhinum ramosissimum (Wall.) Betsche: A traditionally important medicinal plant. Kuwait Journal of Science. 2021. Vol. 48, no. 3, pp.1-10.
https://search.emarefa.net/detail/BIM-1503288
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 8-10
Record ID
BIM-1503288