Spectrophotometric Assay of chloramphenicol in dosage via dioazotisation and coupling with 2,5-dimethyl phenol

Abstract EN

In view of the importance of the medicinal compound Chloramphenicol (CAP)under current study and its uses in the treatment of many pathological conditions.

An easy and accurate spectrophotometric method was proposed for the determination of the drug compound in its free form and in its pharmaceutical preparations.

The method is based on reducing the nitro group in the Chloramphenicol to the amine and then diazotization of the amine group using nitroso acid prepared simultaneously inside the solution using acidic sodium nitrite.

Then coupling of the diazonum salt of CAP with 2,5- dimethyl phenol in a basic medium to give a colored product (Azo dye) The azo dye gives the highest absorption at 453 nm, the Beer's law applied from 1 to 13 μgCAP /ml the method is characterized by a good accuracy and precision, as it reached the values of -2.42 to 0.54% and not more than 1.3050% respectively.

Also, the method was highly sensitive and acceptable through the values of molar absorption and Sandell's significance, and their values were 2.775x104 l/.mol.cm.

and 0.0116 μg/cm2 sequentially.

The research contained the application of the proposed method in estimating CAP in its pharmaceutical formulation (eye drops) with clinically acceptable results od gave accepted analytical results.