Isolation and identification of grapevine trunk diseases in Palestine and possible use of bacteria as biocontrol agents against the disease
Other Title(s)
عزل و تشخيص أمراض جذع العنب في فلسطين و إمكانية استخدام البكتيريا في المكافحة الحيوية
Joint Authors
Source
Palestine Technical University Kadoorie Research Journal
Issue
Vol. 11, Issue 2 (30 Jun. 2023), pp.88-98, 11 p.
Publisher
Palestine Technical University-Kadoorie
Publication Date
2023-06-30
Country of Publication
Palestine (West Bank)
No. of Pages
11
Main Subjects
Abstract AR
يعتبر مرض جذع العنب (GTD) تهديدا حقيقيا لإنتاج العنب.
هدفت هذه الدراسة إلى التعرف على العوامل المسببة لهذا المرض في بساتين العنب الفلسطينية و تقييم فاعلية البكتيريا لمكافحته في الظروف المخبرية.
توصلت نتائج هذه الدراسة إلى عزل الفطريات المسببة والتي تم تشخيصها باستخدام ال PCR حيث تم تعريفها حسب بنك الجينات على أنها Fusarium solani GR و Neofusicoccum parvum GR3.
تم بعد ذلك دراسة تأثير خمسة أنواع من البكتيريا على نمو هذه الفطريات.
و أثبتت النتائج إلى أن العزلات البكتيرية Pseudomonas fluorescence ORS3 و Pseudomonas fluorescence PFL لديها القدرة على تثبيط نمو الفطريات اكثر من غيره من العزلات الأخرى.
و قد كان تأثير البكتيريا على فطر F.
solani GR أكبر من تأثيره على العزلة الفطرية N.
parvum GR3.
و حسب معلوماتنا فان هذه الدراسة هي الأولى من نوعها التي تم فيها تشخيص مسببات أمراض جذع العنب فب فلسطين.
و عليه فان المزيد من الدراسات يجب إجراؤها لاختبار فعالية العزلات البكتيرية في ظل الظروف الحقلية.
Abstract EN
The grapevine trunk disease (GTD) has been considered a real threat on grape production.
This study aimed to identify the GTD causing agents in Palestinian grapevine orchards and to evaluate the efficacy of bacteria to control the disease under in vitro conditions.
Two fungal isolates; Fusarium solani isolate GR and Neofusicoccum parvum isolate GR3 were identified and diagnosed using PCR and BLASTn analysis.
After that, five bacterial isolates were tested for their efficacy against the growth of the fungi.
Pseudomonas fluorescence isolate ORS3 and Pseudomonas fluorescence isolate PFL showed very strong inhibition zones (> 10 mm, ++++) against both fungi under in vitro conditions.
The other bacterial isolates were able to inhibit the fungi but the inhibition was less and varied among the bacteria.
The effect of the bacteria on F.
solani isolate GR was greater than that on N.
parvum isolate GR3.
Up to our knowledge, this study was the first of its kind in Palestine that identifies GTD.
Further studies are needed to test the efficacy of the bacterial isolates under field conditions.
American Psychological Association (APA)
Durubi, Maryam& Salman, Mazin. 2023. Isolation and identification of grapevine trunk diseases in Palestine and possible use of bacteria as biocontrol agents against the disease. Palestine Technical University Kadoorie Research Journal،Vol. 11, no. 2, pp.88-98.
https://search.emarefa.net/detail/BIM-1505232
Modern Language Association (MLA)
Durubi, Maryam& Salman, Mazin. Isolation and identification of grapevine trunk diseases in Palestine and possible use of bacteria as biocontrol agents against the disease. Palestine Technical University Kadoorie Research Journal Vol. 11, no. 2 (2023), pp.88-98.
https://search.emarefa.net/detail/BIM-1505232
American Medical Association (AMA)
Durubi, Maryam& Salman, Mazin. Isolation and identification of grapevine trunk diseases in Palestine and possible use of bacteria as biocontrol agents against the disease. Palestine Technical University Kadoorie Research Journal. 2023. Vol. 11, no. 2, pp.88-98.
https://search.emarefa.net/detail/BIM-1505232
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 97-98
Record ID
BIM-1505232