Indirect spectrophotometric method for determination of niclosamide via oxidation reaction and bleaching colour of methylene blue dye
Joint Authors
Fathi, Sahar A.
Uthman, Nabil S.
al-Tai, Amir Th.
Source
African Journal of Advanced Pure and Applied Sciences
Issue
Vol. 2, Issue 2 (30 Apr. 2023), pp.235-243, 9 p.
Publisher
African Academy of Advanced Studies
Publication Date
2023-04-30
Country of Publication
Libya
No. of Pages
9
Main Subjects
Abstract EN
Simple, delicate and sensitive method for estimation niclosamide (NICA) as pure, pharmaceutical and veterinary formulations.
the method is based on the oxidation of niclosamide (NICA) by using an excess of the oxidizing agent N-bromosuccinimide (NBS) in an acidic medium.
then the unreacted N-bromosuccinimide is reacted with methylene blue (MB) dye where the color of the dye is bleached and measured at the maximum wavelength of the dye 664 nm.
the linear graph of absorbance versus concentration indicates that Beer's law applies within the concentration range 2-6 μg.mL-1 of niclosamide.
the determination coefficient (R2) was found to be 0.9955 and molar absorptivity value was 4.6546x104 L.mol-1 .cm-1and the Sandell's sensitivity value was 0.00702 μg .cm-2 and the quantitative limit attained (LOQ) was 0.0148 μg.mL-1.
the limit of detection (LOD) was 0.0044 μg.mL-1.
with -0.15% to -0.90% relative error and relative standard deviation was 0.65 to 1.93.
American Psychological Association (APA)
Fathi, Sahar A.& Uthman, Nabil S.& al-Tai, Amir Th.. 2023. Indirect spectrophotometric method for determination of niclosamide via oxidation reaction and bleaching colour of methylene blue dye. African Journal of Advanced Pure and Applied Sciences،Vol. 2, no. 2, pp.235-243.
https://search.emarefa.net/detail/BIM-1508458
Modern Language Association (MLA)
Fathi, Sahar A.…[et al.]. Indirect spectrophotometric method for determination of niclosamide via oxidation reaction and bleaching colour of methylene blue dye. African Journal of Advanced Pure and Applied Sciences Vol. 2, no. 2 (Apr. / Jun. 2023), pp.235-243.
https://search.emarefa.net/detail/BIM-1508458
American Medical Association (AMA)
Fathi, Sahar A.& Uthman, Nabil S.& al-Tai, Amir Th.. Indirect spectrophotometric method for determination of niclosamide via oxidation reaction and bleaching colour of methylene blue dye. African Journal of Advanced Pure and Applied Sciences. 2023. Vol. 2, no. 2, pp.235-243.
https://search.emarefa.net/detail/BIM-1508458
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 242-243
Record ID
BIM-1508458