Studying the effect of the genotypes of the GH gene on blood parameters and some structural characteristics of Iraqi buffalo milk

Abstract EN

The samples of this study were collected from the private fields of buffalo breeders in the Al-Tar district of Dhi Qar Governorate / Karma Bani Said district, where 50 samples of milking female buffaloes were used in the experiment, with ages ranging from (2-3 years), this study was conducted in the laboratories of the College Agriculture and Marshes Department of Animal Production for the period from 15/11/2021 to 30/5/2022 with the aim of diagnosing the genetic morphology of the growth hormone gene in Iraqi buffaloes and its relationship to blood parameters and some characteristics of milk composition.

Where the blood parameters measurements included the number of red blood cells, the number of white blood cells, the percentage of hemoglobin in the blood and the volume of the aggregated cells.

As for the measurement of milk components, it included protein percentage, lactose sugar percentage, fat percentage, percentage of non-fat solids, milk density, freezing point, ash percentage and water percentage.

Then DNA was extracted from animal blood samples in the Molecular Genetics Laboratory of For the Marshes Research Center / Dhi Qar University, the primer of the growth hormone gene was amplified and then the genotypes in the growth hormone gene were determined using (PCR) technique by electrophoresis with a relay device, and then the sequence of bases was analyzed using the sanger sequencing technique.

And then study the relationship between the genetic structures and the traits mentioned above.