The impact of various antioxidant supplementation on ram's sperm quality, fertilization, and early embryo development, in vitro

Abstract EN

The in vitro embryo production (IVEP) is very stressful for gametes.

Gametes are subjected during in vitro manipulation to many different types of stress; oxidative stress is the most prominent one, which will cause damage or alter the genetic material of the sperm and reduce the quality of the oocytes, and has a crucial impact on the possibility of developing embryos even after implantation.

This study aimed to determine the influence of antioxidants on the achievement of In vitro culture (IVC) and sperm's ability to adhere to and penetrate further into In vitro maturated oocytes.

For this purpose, we have incubated ram sperm using four different treatments in terms of antioxidants: melatonin, cysteamine, vitamin C, and vitamin E.

They were incubated by the standard methods of maturation and capacitation of sperm.

The oocytes were fertilized by spermatozoa that had been capacitated with two groups of fertilization media, the first group containing melatonin and the second group containing cysteamine.

Compared with other groups, sperms treated with melatonin demonstrated hyperactivity, and the fertilization rate was significantly increased.

As for the IVF medium containing melatonin, it was superior to cysteamine in embryo development rates.

In conclusion, melatonin could be a promising tool for improving sperm competence for fertilizing oocytes and embryo development in sheep.