Effect of selenium nanoparticles against protoscoleces of Echinococcus granulosus in vitro and hydatid cysts in mice

Abstract EN

This study determined the influence of selenium nanoparticles on the vitality of the protoscoleces of Echinococcus granulosus in vitro; seven concentrations were used : 50, 100, 150, 200, 250, 350, and 500 µg/ml for different exposure times : ten, twenty, thirty, and sixty minutes, respectively.

Albino mice Mus musculus were injected with protoscoleces exposed to nanoparticles at 100, 150, and 200 µg/ml concentrations for 60 minutes.

In contrast, control groups were injected with non-exposed protoscoleces.

Mice were dissected three, four-, and five-months post infestation.

Many criteria were relied on : numbers, weights, diameters of growing hydatid cysts, and their reduction proportion.

The outcomes uncovered an apparent influence of selenium nanoparticles on the viability of protoscoleces of Echinococcus granlosus by the increase in exposure time in vitro, as well as diminish in the numbers of the larvae in processed mice versus the unprocessed collection; no cysts evolved inside processed mice at the concentration 200 µg/ml, three and four-months post-infection.

In contrast, the reduction rate was 90% in mice injected with exposed protoscoleces at the concentration of 150 µg/ml next 4 and 5 months of infection.