Induction of defense responses in faba bean plants against chocolate spot disease caused by botrytis fabae using chitosan
Other Title(s)
استحثاث الاستجابات الدفاعية في نباتات الفول البلدي ضد مرض التبقع الشيكولاتي المتسبب عن فطر fabae Botrytis باستحداث الشيتوزان
Joint Authors
Bayyumi, Shukri Ramadan
Id, Asya Rashad
Amir, Ghunaym Muhammad
Source
Journal of Agricultural and Environmental Sciences
Issue
Vol. 20, Issue 3 (31 Dec. 2021), pp.32-63, 32 p.
Publisher
Damanhour University Faculty of Agriculture
Publication Date
2021-12-31
Country of Publication
Egypt
No. of Pages
32
Main Subjects
Topics
Abstract EN
Chocolate spot disease caused by Botrytis fabae is a major problem that limits faba bean production in Egypt.
The in vitro antifungal activity of chitosan and its ability to elicit defense responses in two faba bean cultivars Giza 416 (resistant) and Giza 40 (susceptible) against this fungus were evaluated.
Seven isolates of B.
fabae were cleaned from infected leaves.
All isolates were pathogenic to Giza 40 cultivar, with isolate 3 (Bf-3) was the most virulent.
Chitosan inhibits the mycelial growth of B.
fabae (Bf-3) in concentration-dependent manner.
At 0.75 % and 1 % , chitosan completely inhibits the growth of B.
fabae.
Under greenhouse conditions, two fab bean cultivars, Giza 416 (resistant) and Giza 40 (susceptible) were sprayed with 0.5% chitosan.
Treatment with chitosan 0.5% reduced the chocolate spot disease severity for both cultivars compared with untreated infected control.
Plants of both cultivars treated with chitosan showed a lower rate of lipid peroxidation and higher levels of chlorophyll and total phenols compared with untreated infected control.
Chitosan stimulated the activity of defense enzymes such as peroxidase (POD), polyphenol oxidase (PPO), and phenylalanine ammonia-lyase (PAL).
Furthermore, chitosan treatment increased the expression levels of three pathogenesis related (PR) genes including PR1, PR2 (β-1, 3-glucanase), and chitinase.
The resistant cultivar (Giza 461), whether treated or untreated with chitosan displayed weaker symptoms, and enhanced values for all physiological and molecular responses than the susceptible cultivar (Giza 40).
The findings show that chitosan has a high potential for protecting faba bean plants from B.
fabae by regulating biochemical, physiological and molecular responses in faba bean plants under infection
American Psychological Association (APA)
Bayyumi, Shukri Ramadan& Id, Asya Rashad& Amir, Ghunaym Muhammad. 2021. Induction of defense responses in faba bean plants against chocolate spot disease caused by botrytis fabae using chitosan. Journal of Agricultural and Environmental Sciences،Vol. 20, no. 3, pp.32-63.
https://search.emarefa.net/detail/BIM-1542860
Modern Language Association (MLA)
Bayyumi, Shukri Ramadan…[et al.]. Induction of defense responses in faba bean plants against chocolate spot disease caused by botrytis fabae using chitosan. Journal of Agricultural and Environmental Sciences Vol. 20, no. 3 (Dec. 2021), pp.32-63.
https://search.emarefa.net/detail/BIM-1542860
American Medical Association (AMA)
Bayyumi, Shukri Ramadan& Id, Asya Rashad& Amir, Ghunaym Muhammad. Induction of defense responses in faba bean plants against chocolate spot disease caused by botrytis fabae using chitosan. Journal of Agricultural and Environmental Sciences. 2021. Vol. 20, no. 3, pp.32-63.
https://search.emarefa.net/detail/BIM-1542860
Data Type
Journal Articles
Language
English
Notes
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Record ID
BIM-1542860