Estimation of phenolic constituents of asclepias sinaicus (muschl.)‎

Abstract EN

Asclepius sinuous (Muschl.) was collected from natural habitat (El- Quasimodo road at north Sinai) during the year of 2005.

It is known in Arabic as ghalquit el-deeb or herbal or gheil.

Nine main flavonoids were isolated from the ethologic extract of the plant aerial parts and three from the plant seeds.

They were established by Nuclear magnetic resonance for hydrogen proton and carbon thirteen (1H and 13C-NMR) and different spectral data.

Plant aerial parts contain quercetin, ruin, quercetin-3- galactopyranoside, quercetin-3-glucoside-7-rhamnoside-3 / meth oxide, kaempferol, myricetin, myricetin 3- rutinoside, isorhamnetin and isorhamnetin 3-rutinoside.

While plant seeds contain rutin, quercetin-3- galactopyranoside and kaempferol> The aerial parts contain the coumarone compounds ; masculine and scopolamine while plant seeds contain scopolamine.

A quantitative estimation of the total flavonoids in the alcoholic extracts of both aerial parts and seeds was accomplished by spectrophotometric method during the four seasons of the year (2005).

The percentage of total flavonoids of aerial parts reached maximum value of 3.96 % collected in spring season.

Qualitative and quantitative estimation of the phenolic constituents were determined using HPLC techniques.

There are fifteen and ten compounds identified in aerial parts and seeds of A.

sinuous, respectively.

The highest concentration were p-comedic acid (1.359 and 0.419 μgm / 100gm) for plant aerial parts and seeds, respectively.