Simultaneous determination of captopril and indapamide in pharmaceuticals and human plasma by capillary electrophoresis

Abstract EN

Capillary electrophoresis (CE) with UV photo diode-array detector was utilized to adopt a new method for the assay of captopril and indapamide in pharmaceuticals and human plasma.

Electrophoretic conditions controlling the analysis were optimized to develop separation, sensitivity and rapidity.

The optimum conditions obtained were 100 mM borate at pH 9.0, injection time 10.0 s, voltage 25 kV and column temperature 25C with detection at 220 nm.

Wide dynamic range (1-100 μg ml-1) for both drugs was obtained.

In addition, good recovery (92.8-99.6 %) in pharmaceutical formulation and human plasma matrices were gained.

The method also recorded good repeatability (RSD ≤ 1.2, n = 7) and good intermediate precision (RSD ≤ 2.3, n = 5).

The sensitivity of the method was developed by the optimization and the pre-concentration conducted for human plasma sample using liquid-liquid extraction.

The limit of detection gained (0.075 and 0.045 μg ml-1 for captopril and indapamide, respectively) reached the level of both drugs possibly found in human plasma.

The method is suitable to be applied in pharmaceutical industries for quality control purpose and clinical laboratory for therapeutic drug monitoring purpose.