Foot and mouth disease (fmd)‎ in egypt 2010

Abstract EN

Eight Egyptian governorates were involved in this study representing Lower, Med and Upper Egypt ( Ismailia, Dakahlia, Monofia, Sharkia, Helwan, Giza, Beni Suef and Menya).

Eight hundred randum serum samples were collected from cattle (hundred sample (from each governorate) .

Eighty virological samples were collected (for virus isolation) on the base of clinical signs FMD (salivation ( or epithelial tongue eruption ).

The collected materials (samples) were submitted for diagnosis and epidemiological monitoring of circulating FMD virus strains in (cattle in) Egypt.

The serum samples were examined for the circulating antibodies against FMDV serotypes (O1 & A) by using ELISA.

Prio CHECK FMDV Nonstructural protein blocking ELISA was adopted to differentiate between vaccinated and antibodies produced from previously infected cattle.

The virological samples for virus isolation were inoculated in unweand baby mice and primary calf kidney cell culture for isolation of FMDV.

The positive samples were checked by ELISA test to differentiate between O1 &A FMDV serotypes.

The results showed that, the circulating antibodies (in cattle) are present moderately in the 8 governorates ; the Priocheck blocking ELISA indicated that, about 20 % of these antibodies were due to previous infection, while 68 % due to vaccination by FMD bivalent locally produced inactivated (FMD) vaccine.

The virological investigation indicated that, FMDV serotypes O1&A still existing and circulating in Egyptian cattle.