Detection and inactivation of enterobacter Sakazakii (cronobacter)‎ in powdered infant milk formula

Abstract EN

-E.

sakazakii is considered as an opportunistic bacterium in elderly people and infants.

Epidemiological studies implicate dried infant formula as the primary source of transmission of this pathogen.

A total of 177 powdered infant milk formula (PIMF) [39 low birth weight formula from 1 day: 6 months (LBWF), 63 infant milk formula for infant below 6 months (IMF), 55 follow-on formula from 6:12 months and 20 growing children formula For age 1:4 years).

The samples were collected from different pharmacies in Kafr El-Sheikh governorate, Egypt, within the accurate shelf life period, i and then transferred to the laboratory in their packages to be tested for detection of E.

Sakazakii and detection of the efficacy of Lysozyme and thermally modified lysozyme for inactivating E.

Sakazakii in reconstituted infant formula at different storage temperatures.

The results revealed that E.

Sakazakii could be detected in 5.1, 6.3 and 5% of examined LBWF, IMF and growing children formula respectively with total percentage of 3.95%, • but the organism could not be detected in follow-on formula.

The results;; indicated that thermally modified lysozyme, was more effective than Lysozyme in inhibition of E.

Sakasakii growth at 4 °C (p <0.001), thermally Modified lysozyme had more inhibitory effect on e.

Sakasakii at 4 °C than At 25and 37 °c.

In conclusion the combined efforts of public health and " regulatory officials, as well as manufacturers, were considered important aspects of the management of risks associated with disease causing e.

Sakazakii in PIMF, also the uses of thermally modified lysozyme can , exert a significant inhibitory activity against this organism in reconstituted milk formula specially when kept at refrigeration temperature.