Detection of trypanosoma evansi in camels using syber green1 real time PCR assay

Abstract EN

Camel Trypanosomasis caused by Trypanosoma evansi is still a serious problem in camel husbandry which causes considerable losses in many camel-rearing regions of the world.

In the present study, 75 camels clinically suspected for surra were examined parasitologicaly by giemsa Stained blood smear and for DNA amplification by real time PCR.

Giemsa stained blood smears on microscopical examination detected only 9 out of 75 camels (12%) as positive for the presence of T.

Evansi.

While, out of 75 DNA samples prepared from camel blood, 72 were positive by real time PCR assay (96%).

Primers (TR3, TR4) derived from nuclear repetitive gene of T.

Evansi and syber greenl fluorescent dye has been used.

The melting peak chart of the positive samples showed one single peak at an average TM of 85.0°c.

Application of this real time PCR to clinical samples resulted in rapid, direct detection of T.

Evansi in blood of naturally infected camels.

The described real time PCR provides a valuable tool to study the epidemiology of T.

Evansi infection in camels and other susceptible animal population.