Spectrophotometric determination of mexiletine hydrochloride in pharmaceutical preparations, urine and serum using complexing reagents
Joint Authors
Najib, Fadil M.
Abd Allah, Ahmad M.
Salh, Dler M.
Source
Issue
Vol. 2010, Issue 37 (31 Mar. 2010), pp.35-50, 16 p.
Publisher
University of Babylon College of Sciences
Publication Date
2010-03-31
Country of Publication
Iraq
No. of Pages
16
Main Subjects
Pharmacy, Health & Medical Sciences
Chemistry
Topics
Abstract AR
في ها العمل تم التقدير الطيفي ل ( MH ) ( Mexiletine Hydrochloride ) [1-methyl-2-(2,6-xylyloxy) ethylamine hydrochloride] باستعمال الكواشف (المثيل البرتقالي (MO) و الزايلنول البرتقالي (XO)).
الطريقة تتضمن إضافة 1.5 ml من 0.1% MO أو 1.2 ml من 0.05% XO لمقدار معين من MH, القياس أو النماذج, محتويا ما بين (5-20 μg.
ml-1) يرج المزيج لمدة (30 s).
ثم يخفف بالماء المقطر إلى ما يقارب 23 ml في حالة ال mo و 8 ml في حالة XO في قناني حجمية.
يضبط ال (pH) إلى 2.8 بإضافة ال 1 ml Phthalate Buffer إلى مزيج ال MO ثم يكمل الحجم إلى 25 ml, أو بواسطة HCl و NaOH إلى pH = 5.5 ثم التكملة إلى 10 ml لمزيج ال XO.
ينقل ال (Ion-Pair) الملون المتكون بين ال (MH) و الكواشف إلى قناني الفصل و يستخلص مستعملا CH2Cl2 5.5 ml مع الرج المستمر لمدة (30 - 60 s) ثم يفصل الطبقة العضوية أو المائية لرسم منحنيات المعايرة و القياسات الطيفية ل MH في الأطوال الموجية (429 nm) و (438 nm) في حالتي (MO) و (XO) على التوالي.
محاليل البلانك تجري لها نفس العمليات تماما و بدون ال MH.
قيم الامتصاص الجزيئي (ε L.mol-1.cm-1), وحد الكشف و حد الاستقامة (μg.ml-1) و (r2), كانت على التعاقب 4.2 * 103, 0.35, 4 و 0.9961 في حالة ال (MH-MO) و 2.3 * 104, 1.35, 5 و 0.9961 في حالة ال MH-XO.
لقد استخدمت الطريقتين بدقة مناسبة تراوحت بين (%E = 1.6-3) و (%S.D = 3-4) لتقدير ال MH في نماذج محضرة و نماذج الدم و الإدرار الحقيقيين.
Abstract EN
In this work mexiletine hydrochloride (MH) [1-(2, 6- (dimethylphenoxy) -2- aminopropane hyrochloride] has been determined spectrophotometrically, using methyl orange (MO) and xylenol orange (XO).
The method involved the addition of 1.5ml 0.1 % (MO) or 1.2ml 0.05 % (XO) reagents to a certain amount of MH, standard or samples, containing between (5-20 μgml-1) MH.
The mixture is shaken for (30 sec.) and diluted to about 23ml in case of MO and to 8ml in case of XO in volumetric flasks using distilled water.
The pH was adjusted by adding 1ml phthalate buffer pH 2.8 to the MO mixture and finally completed to 25ml, or with NaOH and HCl to pH 5.5 in case of XO and completed to 10ml.
The colored ion-pair formed between MH and the reagents were transferred into separating funnels and extracted using 5.5ml CH2Cl2 and were shaken for 30–60s.
After separation, the organic or aqueous layers were used for constructing calibration curves for spectrophotometric measurements of MH at 429nm and 438nm in cases of MO and XO respectively.
The blanks were carried out in exactly the same way throughout the whole procedure.
Molar absorptivity (ε L.
mol-1.
cm-1), detection limit, limit of linearity (μg.
ml-1) and r2 were : 4.2 x 103, 0.32, 4 and 0.9961 for (MH-MO) and 2.3 x 103, 1.35, 5 and 0.9961 for (MHXO) respectively.
The method was used with reasonable accuracy and precision of (1.6-3.6 E %) and ( ± 1-3.6 S.D %) respectively, for the determination of (MH) in synthetic samples of real blood, urine and capsules.
American Psychological Association (APA)
Najib, Fadil M.& Abd Allah, Ahmad M.& Salh, Dler M.. 2010. Spectrophotometric determination of mexiletine hydrochloride in pharmaceutical preparations, urine and serum using complexing reagents. National Journal of Chemistry،Vol. 2010, no. 37, pp.35-50.
https://search.emarefa.net/detail/BIM-240873
Modern Language Association (MLA)
Najib, Fadil M.…[et al.]. Spectrophotometric determination of mexiletine hydrochloride in pharmaceutical preparations, urine and serum using complexing reagents. National Journal of Chemistry No. 37 (2010), pp.35-50.
https://search.emarefa.net/detail/BIM-240873
American Medical Association (AMA)
Najib, Fadil M.& Abd Allah, Ahmad M.& Salh, Dler M.. Spectrophotometric determination of mexiletine hydrochloride in pharmaceutical preparations, urine and serum using complexing reagents. National Journal of Chemistry. 2010. Vol. 2010, no. 37, pp.35-50.
https://search.emarefa.net/detail/BIM-240873
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 49-50
Record ID
BIM-240873